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Arecoline Induces ROS Accumulation, Transcription of Proinflammatory Factors, and Expression of KRT6 in Oral Epithelial Cells.
Wang, Tong-Hong; Shen, Yen-Wen; Chen, Hsin-Ying; Chen, Chih-Chieh; Lin, Nan-Chin; Shih, Yin-Hwa; Hsia, Shih-Min; Chiu, Kuo-Chou; Shieh, Tzong-Ming.
Afiliação
  • Wang TH; Biobank, Chang Gung Memorial Hospital, Taoyuan 33305, Taiwan.
  • Shen YW; School of Dentistry, China Medical University, Taichung 404328, Taiwan.
  • Chen HY; School of Dentistry, China Medical University, Taichung 404328, Taiwan.
  • Chen CC; Department of Sports Medicine, China Medical University, Taichung 404328, Taiwan.
  • Lin NC; School of Dentistry, China Medical University, Taichung 404328, Taiwan.
  • Shih YH; Department of Oral and Maxillofacial Surgery, Show Chwan Memorial Hospital, Changhua 505029, Taiwan.
  • Hsia SM; Department of Oral and Maxillofacial Surgery, Changhua Christian Hospital, Changhua 500011, Taiwan.
  • Chiu KC; Department of Healthcare Administration, Asia University, Taichung 41354, Taiwan.
  • Shieh TM; School of Nutrition and Health Sciences, College of Nutrition, Taipei Medical University, Taipei 110301, Taiwan.
Biomedicines ; 12(2)2024 Feb 09.
Article em En | MEDLINE | ID: mdl-38398015
ABSTRACT
Areca nut is a major contributor to the high prevalence of oral cancer in Asia. The precise mechanisms by which areca nut stimulates mucosal cells and contributes to the progression of oral cancer urgently require clarification. The current study aimed to assess the effects of arecoline on the normal human gingival epithelium cell line S-G. Cell viability, levels of reactive oxygen species (ROS), protein expression, cellular morphology, and gene expression were evaluated using the MTT test, flow cytometry, Western blot analysis, optical or confocal microscopy, and RT-qPCR. Keratin (KRT6) analysis involved matched normal and cancer tissues from clinical head and neck specimens. The results demonstrated that 12.5 µg/mL of arecoline induced ROS production, tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) mRNA expression in S-G cells. This activation of the MAPK/ERK pathway increased KRT6 expression while limiting cell migration. In head and neck cancer tissues, KRT6B gene expression exceeded that of normal tissues. This study confirms that arecoline induces ROS accumulation in normal cells, leading to the secretion of proinflammatory factors and KRT6 expression. This impedes oral mucosal healing, thereby promoting the progression of oral cancer.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2024 Tipo de documento: Article