Your browser doesn't support javascript.
loading
Analysis of Proliferation, Apoptosis, and Motility in Mouse Embryonic Melanocytic Precursor Cells.
Crawford, Melissa; Barr, Kevin; Dagnino, Lina.
Afiliação
  • Crawford M; Department of Physiology and Pharmacology, Child Health Research Institute and Lawson Health Research Institute, University of Western Ontario, London, ON, Canada.
  • Barr K; Department of Physiology and Pharmacology, Child Health Research Institute and Lawson Health Research Institute, University of Western Ontario, London, ON, Canada.
  • Dagnino L; Department of Physiology and Pharmacology, Child Health Research Institute and Lawson Health Research Institute, University of Western Ontario, London, ON, Canada. ldagnino@uwo.ca.
Methods Mol Biol ; 2849: 135-148, 2024.
Article em En | MEDLINE | ID: mdl-38441720
ABSTRACT
In this chapter, we provide a method to purify and culture embryonic melanocytic stem cells that express green fluorescent protein in a cell-type specific manner. Isolation of melanocytic lineage cell populations that are >98% pure is accomplished through the use of GFP-based fluorescence activated cell sorting. We also provide a method to culture the purified melanoblasts and to analyze their proliferation, apoptosis, and motility properties.
Assuntos
Palavras-chave
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Movimento Celular / Apoptose / Proliferação de Células / Melanócitos Limite: Animals Idioma: En Ano de publicação: 2024 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Movimento Celular / Apoptose / Proliferação de Células / Melanócitos Limite: Animals Idioma: En Ano de publicação: 2024 Tipo de documento: Article