Application of MicroResp™ for quick and easy detection of plastic degradation by marine bacterial isolates.

ABSTRACT

Microplastic debris in the marine environment is a global problem. Biodegradable polymers are being developed as alternatives to petroleum-based plastics, and quick and easy methods for screening for bacterial strains that can degrade such polymers are needed. As a screening method, the clear zone method has been widely used but has technical difficulties such as plate preparation and interpretation of results. In this study, we adapted the MicroResp™ system to easily detect biodegradation activity of marine bacteria in a 3-day assay. Among the 6 bacterial strains tested, 3, 2 and 1 strain degraded poly (butylene succinate-co-adipate) (PBSA), poly (ε-caprolactone) (PCL) and poly (3-hydroxybutyrate-co-3-hydroxyhexanoate), respectively. Only one strain that showed degradation activity of PBSA and PCL in the MicroResp™ system was also positive in the clear zone assay on the respective emulsion plates. Our results show that the adapted MicroResp™ system can screen for bacterial strains that degrade plastic.