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Mesenchymal Stromal Cells from Human Wharton's Jelly Modulate the Intraocular Immune Response in a Glucocorticoid Hypertension Model: An Exploratory Analysis.
Dos Santos Evangelho, Karine; Cifuentes-González, Carlos; Rojas-Carabali, William; De Vivero-Arciniegas, Clemencia; Cañas-Arboleda, Mariana; Salguero, Gustavo; Ramírez-Santana, Carolina; de-la-Torre, Alejandra.
Afiliação
  • Dos Santos Evangelho K; Doctoral Program Biomedical and Biological Sciences, School of Medicine and Health Sciences, University of Rosario, Bogota, Colombia, evangelho.karine@gmail.com.
  • Cifuentes-González C; Neuroscience (NEUROS) Research Group, Neurovitae Center for Neuroscience, Institute of Translational Medicine (IMT), School of Medicine and Health Sciences, University of Rosario, Bogota, Colombia.
  • Rojas-Carabali W; Neuroscience (NEUROS) Research Group, Neurovitae Center for Neuroscience, Institute of Translational Medicine (IMT), School of Medicine and Health Sciences, University of Rosario, Bogota, Colombia.
  • De Vivero-Arciniegas C; Higher School of Ophthalmology Barraquer Institute of America, Bogota, Colombia.
  • Cañas-Arboleda M; Advanced Therapies Unit, Instituto Distrital de Ciencia Biotecnología e Innovación en Salud-IDCBIS, Bogota, Colombia.
  • Salguero G; Advanced Therapies Unit, Instituto Distrital de Ciencia Biotecnología e Innovación en Salud-IDCBIS, Bogota, Colombia.
  • Ramírez-Santana C; Center for Autoimmune Diseases Research (CREA), Institute of Translational Medicine (IMT), School of Medicine and Health Sciences, University of Rosario, Bogota, Colombia.
  • de-la-Torre A; Neuroscience (NEUROS) Research Group, Neurovitae Center for Neuroscience, Institute of Translational Medicine (IMT), School of Medicine and Health Sciences, University of Rosario, Bogota, Colombia.
Ophthalmic Res ; 67(1): 232-247, 2024.
Article em En | MEDLINE | ID: mdl-38447539
ABSTRACT

INTRODUCTION:

Glaucoma is a neurodegenerative disease characterized by the loss of retinal ganglion cells. Recent research suggests immunological changes such as cytokine imbalance may affect its pathophysiology. This implies that immunomodulation, like that of mesenchymal cells, could be a potential therapeutic avenue for this disease. However, the effects of intravitreal injections of human Wharton's jelly-derived mesenchymal stromal cells (hWJ-MSCs) on intraocular immune response have not been assessed in ocular hypertension (OH) models.

METHODS:

We explored this by measuring cytokine levels and expression of other markers, such as glial fibrillary acidic protein (GFAP) and T cells, in 15 randomly divided New Zealand rabbits G1 OH, G2 hWJ-MSCs, and G3 OH+hWJ-MSCs. We analyzed the aqueous humor (IL-6, IL-8, and TNF-α) and vitreous humor (IFN-γ, IL-10, and TGF-ß) using ELISA and flow cytometry (cell populations), as well as TCD3+, TCD3+/TCD4+, and TCD3+/TCD8+ lymphocytes, and GFAP in the retina and optic nerve through immunohistochemistry.

RESULTS:

We found a decrease in TNF-α, IL-6, IFN-γ, IL-10, and IL-8 in G3 compared to G1 and an increase in TGF-ß in both G2 and G3. TCD3+ retinal infiltration in all groups was primarily TCD8+ rather than TCD4+ cells, and strong GFAP expression was observed in both the retina and optic nerves in all groups.

CONCLUSION:

Our results suggest that cellular and humoral immune responses may play a role in glaucomatous optic neuropathy and that intravitreal hWJ-MSCs can induce an immunosuppressive environment by inhibiting proinflammatory cytokines and enhancing regulatory cytokines.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ensaio de Imunoadsorção Enzimática / Hipertensão Ocular / Citocinas / Modelos Animais de Doenças / Geleia de Wharton / Células-Tronco Mesenquimais Limite: Animals / Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ensaio de Imunoadsorção Enzimática / Hipertensão Ocular / Citocinas / Modelos Animais de Doenças / Geleia de Wharton / Células-Tronco Mesenquimais Limite: Animals / Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article