One-step rapid tracking and isolation of senescent cells in cellular systems, tissues, or animal models via GLF16.
STAR Protoc
; 5(1): 102929, 2024 Mar 15.
Article
em En
| MEDLINE
| ID: mdl-38460134
ABSTRACT
Identification and isolation of senescent cells is challenging, rendering their detailed analysis an unmet need. We describe a precise one-step protocol to fluorescently label senescent cells, for flow cytometry and fluorescence microscopy, implementing a fluorophore-conjugated Sudan Black-B analog, GLF16. Also, a micelle-based approach allows identification of senescent cells in vivo and in vitro, enabling live-cell sorting for downstream analyses and live in vivo tracking. Our protocols are applicable to cellular systems, tissues, or animal models where senescence is present. For complete details on the use and execution of this protocol, please refer to Magkouta et al.1.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Senescência Celular
/
Corantes Fluorescentes
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article