Porphyrin-BODIPY Dyad: Enhancing Photodynamic Inactivation via Antenna Effect.
Chembiochem
; 25(9): e202400138, 2024 May 02.
Article
em En
| MEDLINE
| ID: mdl-38478375
ABSTRACT
A porphyrin-BODIPY dyad (P-BDP) was obtained through covalent bonding, featuring a two-segment design comprising a light-harvesting antenna system connected to an energy acceptor unit. The absorption spectrum of P-BDP resulted from an overlap of the individual spectra of its constituent parts, with the fluorescence emission of the BODIPY unit experiencing significant quenching (96 %) due to the presence of the porphyrin unit. Spectroscopic, computational, and redox investigations revealed a competition between photoinduced energy and electron transfer processes. The dyad demonstrated the capability to sensitize both singlet molecular oxygen and superoxide radical anions. Additionally, P-BDP effectively induced the photooxidation of L-tryptophan. In suspensions of Staphylococcus aureus cells, the dyad led to a reduction of over 3.5â
log (99.99 %) in cell survival following 30â
min of irradiation with green light. Photodynamic inactivation caused by P-BDP was also extended to the individual bacterium level, focusing on bacterial cells adhered to a surface. This dyad successfully achieved the total elimination of the bacteria upon 20â
min of irradiation. Therefore, P-BDP presents an interesting photosensitizing structure that takes advantage of the light-harvesting antenna properties of the BODIPY unit combined with porphyrin, offering potential to enhance photoinactivation of bacteria.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Porfirinas
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Staphylococcus aureus
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Compostos de Boro
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Fármacos Fotossensibilizantes
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article