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Chromene-chromene Schiff base as a fluorescent chemosensor for Th4+ and its application in bioimaging of Caenorhabditis elegans.
Dua, Aastha; Saini, Pratiksha; Goyal, Shiwani; Selvam, Pravinkumar; Ashok Kumar, S K; Thiruppathi, Govindhan; Sundararaj, Palanisamy; Sharma, Harish K; Kumar Ramasamy, Selva.
Afiliação
  • Dua A; Department of Chemistry, M.M Engineering College, Maharishi Markandeshwar (Deemed to be University), Mullana, Ambala 133 207, Haryana, India.
  • Saini P; Department of Chemistry, M.M Engineering College, Maharishi Markandeshwar (Deemed to be University), Mullana, Ambala 133 207, Haryana, India.
  • Goyal S; Department of Chemistry, M.M Engineering College, Maharishi Markandeshwar (Deemed to be University), Mullana, Ambala 133 207, Haryana, India.
  • Selvam P; Department of Chemistry, School of Advanced Sciences, Vellore Institute of Technology, Vellore 632 014, Tamil Nadu, India.
  • Ashok Kumar SK; Department of Chemistry, School of Advanced Sciences, Vellore Institute of Technology, Vellore 632 014, Tamil Nadu, India.
  • Thiruppathi G; Unit of Nematology, Department of Zoology, Bharathiar University, Coimbatore 641 046, Tamil Nadu, India.
  • Sundararaj P; Unit of Nematology, Department of Zoology, Bharathiar University, Coimbatore 641 046, Tamil Nadu, India.
  • Sharma HK; Department of Chemistry, M.M Engineering College, Maharishi Markandeshwar (Deemed to be University), Mullana, Ambala 133 207, Haryana, India.
  • Kumar Ramasamy S; Department of Chemistry, M.M Engineering College, Maharishi Markandeshwar (Deemed to be University), Mullana, Ambala 133 207, Haryana, India. Electronic address: selvachemst@gmail.com.
Methods ; 225: 28-37, 2024 May.
Article em En | MEDLINE | ID: mdl-38485032
ABSTRACT
The manuscript presents the synthesis of a new di-chromene Schiff base (COM-CH) by combining 7-(diethylamino)-2-oxo-2H-chromene-3-carbohydrazide and 4-oxo-4H-chromene-3-carbaldehyde, and its characterization using various analytical techniques. The probe COM-CH functional group contains a hard donor atom that selectively complexes with Th4+ ions. This report investigated COM-CH's sensing ability towards Th4+ chromogenic and fluorogenic methods in ACN H2O (82, v/v) with Th4+ ions. The COM-CH-Th4+ complex was excited at 430 nm, resulting in a bright emission band at 475 nm with a 45 nm Stokes shift. The COM-CH probe demonstrated the highest performance at pH 4.0 to 8.0, with a sensitivity of 18.7 nM. The complex formation of COM-CH with Th4+ was investigated using NMR, FTIR spectrometry, and density functional theory calculations. The COM-CH and Th4+ are bound with 21 stoichiometry and an association constant of 1.92 × 108 M-2. The probe's performance enabled the analysis of monazite sand and water samples for Th4+ content. The probe successfully detected Th4+ content in Caenorhabditis elegans, marking the first Th4+ detection in animal models.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bases de Schiff / Benzopiranos / Caenorhabditis elegans / Corantes Fluorescentes Limite: Animals Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bases de Schiff / Benzopiranos / Caenorhabditis elegans / Corantes Fluorescentes Limite: Animals Idioma: En Ano de publicação: 2024 Tipo de documento: Article