Autophagy activity is increased in the cumulus cells of women with poor ovarian response.
Taiwan J Obstet Gynecol
; 63(2): 205-213, 2024 Mar.
Article
em En
| MEDLINE
| ID: mdl-38485316
ABSTRACT
OBJECTIVE:
To evaluate the autophagy status of cumulus cells (CCs) in women with poor ovarian response (POR). MATERIALS ANDMETHODS:
CCs were divided into normal ovarian response (NOR) group and POR group. The ultrastructure of autophagy was analyzed by transmission electron microscopy (NOR n = 18, POR n = 26). The mRNA and protein of autophagy markers were detected by Quantitative real-time polymerase chain reaction (NOR n = 15, POR n = 19) and Western blotting (NOR n = 41, POR n = 38), respectively.RESULTS:
Transmission electron microscopy demonstrated abundant autophagosomes and even autophagic death in the POR group. There were no differences in LC3 and P62 mRNA expression between the two groups (p > 0.05). The BCL2 mRNA expression was lower in the POR group (p < 0.05). Moreover, the LC3 II/I ratio and the P62 protein expression were significantly higher in the POR group (p < 0.05).CONCLUSIONS:
Autophagy in CCs of POR women is activated and the autophagic flux is blocked. The up-regulation of autophagy in CCs may be related to the pathogenesis of POR.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Autofagia
/
Células do Cúmulo
Limite:
Female
/
Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article