Your browser doesn't support javascript.
loading
Nasopharyngeal metatranscriptomics reveals host-pathogen signatures of pediatric sinusitis.
AbuMazen, Nooran; Chu, Vivian; Hunjan, Manjot; Lobb, Briallen; Lee, Sojin; Kurs-Lasky, Marcia; Williams, John V; MacDonald, William; Johnson, Monika; Hirota, Jeremy A; Shaikh, Nader; Doxey, Andrew C.
Afiliação
  • AbuMazen N; Department of Biology, University of Waterloo, Waterloo, Ontario, Canada.
  • Chu V; Waterloo Centre for Microbial Research, University of Waterloo, Waterloo, Ontario, Canada.
  • Hunjan M; Department of Biology, University of Waterloo, Waterloo, Ontario, Canada.
  • Lobb B; Waterloo Centre for Microbial Research, University of Waterloo, Waterloo, Ontario, Canada.
  • Lee S; Department of Biology, University of Waterloo, Waterloo, Ontario, Canada.
  • Kurs-Lasky M; Waterloo Centre for Microbial Research, University of Waterloo, Waterloo, Ontario, Canada.
  • Williams JV; Department of Biology, University of Waterloo, Waterloo, Ontario, Canada.
  • MacDonald W; Waterloo Centre for Microbial Research, University of Waterloo, Waterloo, Ontario, Canada.
  • Johnson M; University of Pittsburgh School of Medicine, Children's Hospital of Pittsburgh of UPMC, Division of General Academic Pediatrics.
  • Hirota JA; University of Pittsburgh School of Medicine, Children's Hospital of Pittsburgh of UPMC, Division of General Academic Pediatrics.
  • Shaikh N; Division of Infectious Diseases, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA.
  • Doxey AC; University of Pittsburgh School of Medicine, Children's Hospital of Pittsburgh of UPMC, Division of General Academic Pediatrics.
medRxiv ; 2024 Mar 04.
Article em En | MEDLINE | ID: mdl-38496499
ABSTRACT
Acute sinusitis (AS) is the fifth leading cause of antibiotic prescriptions in children. Distinguishing bacterial AS from common viral upper respiratory infections in children is crucial to prevent unnecessary antibiotic use but is challenging with current diagnostic methods. Despite its speed and cost, untargeted RNA sequencing of clinical samples from children with suspected AS has the potential to overcome several limitations of other methods. However, the utility of sequencing-based approaches in analysis of AS has not been fully explored. Here, we performed RNA-seq of nasopharyngeal samples from 221 children with clinically diagnosed AS to characterize their pathogen and host-response profiles. Results from RNA-seq were compared with those obtained using culture for three common bacterial pathogens and qRT-PCR for 12 respiratory viruses. Metatranscriptomic pathogen detection showed high concordance with culture or qRT-PCR, showing 87%/81% sensitivity (sens) / specificity (spec) for detecting bacteria, and 86%/92% (sens/spec) for viruses, respectively. We also detected an additional 22 pathogens not tested for in the clinical panel, and identified plausible pathogens in 11/19 (58%) of cases where no organism was detected by culture or qRT-PCR. We assembled genomes of 205 viruses across the samples including novel strains of coronaviruses, respiratory syncytial virus (RSV), and enterovirus D68. By analyzing host gene expression, we identified host-response signatures that distinguished bacterial and viral infections and correlated with pathogen abundance. Ultimately, our study demonstrates the potential of untargeted metatranscriptomics for in depth analysis of the etiology of AS, comprehensive host-response profiling, and using these together to work towards optimized patient care.

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2024 Tipo de documento: Article