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Cre-LoxP and tamoxifen-induced deletion of ovarian quiescin sulfhydryl oxidase 2 showed disruption of ovulatory activity in mice.
Chen, Shih-Yun; Wang, Tse-En; Lee, Wei-Yun; Yang, Ya-Yi; Lai, Hong-Chun; Matsuda, Fuko; Kosek, Haruhiko; Chen, You-Tzung; Li, Sheng-Hsiang; Tsai, Pei-Shiue.
Afiliação
  • Chen SY; Department of Veterinary Medicine, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei, 10617, Taiwan.
  • Wang TE; Graduate Institute of Veterinary Medicine, National Taiwan University, 10617, Taipei, Taiwan.
  • Lee WY; Graduate Institute of Veterinary Medicine, National Taiwan University, 10617, Taipei, Taiwan.
  • Yang YY; Graduate Institute of Veterinary Medicine, National Taiwan University, 10617, Taipei, Taiwan.
  • Lai HC; Department of Veterinary Medicine, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei, 10617, Taiwan.
  • Matsuda F; Department of Veterinary Medical Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, 113-8657, Japan.
  • Kosek H; Center for Integrative Medical Sciences (IMS), RIEKN, Yokohama, Kanagawa, 230-0045, Japan.
  • Chen YT; Research Center for Developmental Biology and Regenerative Medicine, National Taiwan University, 10617, Taipei, Taiwan.
  • Li SH; Graduate Institute of Medical Genomics and Proteomics, College of Medicine, National Taiwan University, 10055, Taipei, Taiwan.
  • Tsai PS; Department of Medical Research, MacKay Memorial Hospital, 25160, Tamsui, Taiwan.
J Ovarian Res ; 17(1): 66, 2024 Mar 19.
Article em En | MEDLINE | ID: mdl-38504307
ABSTRACT

BACKGROUND:

Quiescin sulfhydryl oxidase 2 (QSOX2) is a flavin adenine dinucleotide-dependent sulfhydryl oxidase that is known to be involved in protein folding, cell growth regulation, and redox state modification through oxidative activities. Earlier studies demonstrated the tissue and cellular localization of QSOX2 in the male reproductive tract, as well as the highly-regulated mechanism of QSOX2 protein synthesis and expression through the coordinated action of testosterone and epididymal-enriched amino acid, glutamate. However, the presence and the functions of QSOX2 in female reproduction are unknown. In this study, we applied the Cre-loxP gene manipulation system to generate the heterozygous and homozygous Qsox2 knockout mice and examined its effects on ovarian function.

RESULTS:

We demonstrated that QSOX2 was detected in the follicle-supporting cells (granulosa and cumulus cells) of ovarian follicles of all stages but was absent in the corpus luteum, suggesting its supportive role in folliculogenesis. In comparison with reproductive organogenesis in wild-type mice, there was no difference in testicular and epididymal structure in male Qsox2 knockout; however, Qsox2 knockout disrupted the regular ovulation process in female mice as a drastic decrease in the formation of the corpus luteum was detected, and no pregnancy was achieved when mating males with homozygous Qsox2 knockout females. RNAseq analyses further revealed that Qsox2 knockout altered critical signaling pathways and genes that are responsible for maintaining ovarian functions.

CONCLUSION:

Our data demonstrated for the first time that Qsox2 is critical for ovarian function in mice.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oxirredutases / Tamoxifeno / Células da Granulosa Limite: Animals Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oxirredutases / Tamoxifeno / Células da Granulosa Limite: Animals Idioma: En Ano de publicação: 2024 Tipo de documento: Article