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The Glycine soja cytochrome P450 gene GsCYP82C4 confers alkaline tolerance by promoting reactive oxygen species scavenging.
Wu, Jinyu; Fang, Yangyang; Xu, Liankun; Jin, Xiaoxia; Iqbal, Anam; Nisa, Zaib Un; Ali, Naila; Chen, Chao; Shah, Anis Ali; Gatasheh, Mansour K.
Afiliação
  • Wu J; Department of Chemistry and Molecular biology, School of Life Science and Technology, Harbin Normal University, Harbin, P.R. China.
  • Fang Y; Department of Chemistry and Molecular biology, School of Life Science and Technology, Harbin Normal University, Harbin, P.R. China.
  • Xu L; Department of Chemistry and Molecular biology, School of Life Science and Technology, Harbin Normal University, Harbin, P.R. China.
  • Jin X; Department of Chemistry and Molecular biology, School of Life Science and Technology, Harbin Normal University, Harbin, P.R. China.
  • Iqbal A; Institute of Molecular Biology and Biotechnology IMBB, The University of Lahore, Lahore, Pakistan.
  • Nisa ZU; Institute of Molecular Biology and Biotechnology IMBB, The University of Lahore, Lahore, Pakistan.
  • Ali N; Institute of Molecular Biology and Biotechnology IMBB, The University of Lahore, Lahore, Pakistan.
  • Chen C; Department of Chemistry and Molecular biology, School of Life Science and Technology, Harbin Normal University, Harbin, P.R. China.
  • Shah AA; Department of Botany, Division of Science and Technology, University of Education, Lahore, Pakistan.
  • Gatasheh MK; Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
Physiol Plant ; 176(2): e14252, 2024.
Article em En | MEDLINE | ID: mdl-38509813
ABSTRACT
Recent studies have demonstrated the crucial role of Cytochrome P450 enzymes (CYPs) in the production of secondary metabolites, phytohormones and antioxidants in plants. However, their functional characterization specifically under alkaline stress remains elusive. CYP82C4 was the key gene screened from a family of wild soybean CYPs in our previous studies. The aim of this present study was to clone the Glycine soja GsCYP82C4 gene and characterize its functions in Arabidopsis and Glycine max. The results showed that the GsCYP82C4 gene displayed a high expression in different plant tissues at mature stages compared to young stages. Further, higher temporal expression of the GsCYP82C4 gene was noted at 6, 12 and 24 h time points after alkali treatment in leaves compared to roots. In addition, overexpression of GsCYP82C4 improved alkaline stress tolerance in Arabidopsis via increased root lengths and fresh biomass and strengthened the antioxidant defense system via a reduction in superoxide radicals in transgenic lines compared to wild type (WT) and atcyp82c4 mutants. Further, the expression levels of stress-related marker genes were up-regulated in GsCYP82C4 OX lines under alkali stress. The functional analysis of GsCYP82C4 overexpression in soybean displayed better hairy root growth, increased fresh weight, higher antioxidant enzyme activities and reduced lipid peroxidation rates in OX lines compared to the soybean WT (K599) line. In total, our study displayed positive roles of GsCYP82C4 overexpression in both Arabidopsis and Glycine max to alleviate alkaline stress via altering expression abundance of stress responsive genes, stronger roots, higher antioxidant enzyme activities as well as reduced rates of lipid peroxidation and superoxide radicals.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Arabidopsis / Fabaceae Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Arabidopsis / Fabaceae Idioma: En Ano de publicação: 2024 Tipo de documento: Article