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Hypothetical protein MAA_07646 is required for stress resistance and pathogenicity in Metarhizium robertsii.
Chen, MingYue; Yu, YaShuai; Tong, YouMin; Wu, Hao; Qu, JiaoJiao; Yang, Yang; Huang, Bo.
Afiliação
  • Chen M; Anhui Provincial Key Laboratory of Microbial Pest Control, Anhui Agricultural University, Hefei, 230036, China.
  • Yu Y; Anhui Provincial Key Laboratory of Microbial Pest Control, Anhui Agricultural University, Hefei, 230036, China.
  • Tong Y; Anhui Provincial Key Laboratory of Microbial Pest Control, Anhui Agricultural University, Hefei, 230036, China.
  • Wu H; School of Life Sciences, Anhui Medical University, Hefei, 230032, China.
  • Qu J; Anhui Provincial Key Laboratory of Microbial Pest Control, Anhui Agricultural University, Hefei, 230036, China.
  • Yang Y; Anhui Provincial Key Laboratory of Microbial Pest Control, Anhui Agricultural University, Hefei, 230036, China.
  • Huang B; Anhui Provincial Key Laboratory of Microbial Pest Control, Anhui Agricultural University, Hefei, 230036, China. bhuang@ahau.edu.cn.
World J Microbiol Biotechnol ; 40(5): 141, 2024 Mar 23.
Article em En | MEDLINE | ID: mdl-38519797
ABSTRACT
Metarhizium robertsii, a vital entomopathogenic fungus for pest management, relies on various virulence-related proteins for infection. Identifying these proteins, especially those with unknown functions, can illuminate the fungus's virulence mechanisms. Through RNA-seq, we discovered that the hypothetical protein MAA_07646 was significantly upregulated during appressorium formation in M. robertsii. In this study, we characterized MAA_07646, finding its presence in both the nucleus and cytoplasm. Surprisingly, it did not affect vegetative growth, conidiation, or chemical tolerance. However, it played a role in heat and UV radiation sensitivity. Notably, ΔMAA_07646 exhibited reduced virulence in Galleria mellonella larvae due to impaired appressorium formation and decreased expression of virulence-related genes. In conclusion, MAA_07646 contributes to thermotolerance, UV resistance, and virulence in M. robertsii. Understanding its function sheds light on the insecticidal potential of M. robertsii's hypothetical proteins.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Metarhizium / Mariposas Limite: Animals Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Metarhizium / Mariposas Limite: Animals Idioma: En Ano de publicação: 2024 Tipo de documento: Article