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Network pharmacology analysis and experimental validation to explore the mechanism of kaempferol in the treatment of osteoporosis.
Dong, Qi; Ren, Guoxia; Li, Yanzhao; Hao, Dingjun.
Afiliação
  • Dong Q; Department of Orthopedics, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China.
  • Ren G; Department of Physical Medicine and Rehabilitation, The Affiliated Hospital of Shaanxi University of Chinese Medicine, Xianyang, Shaanxi, China.
  • Li Y; Department of Physical Medicine and Rehabilitation, Xi'an Chest Hospital, Xi'an, Shaanxi, China.
  • Hao D; Department of Traditional Chinese Medicine, First Clinical Medical College, Shaanxi University of Chinese Medicine, Xianyang, Shaanxi, China.
Sci Rep ; 14(1): 7088, 2024 03 26.
Article em En | MEDLINE | ID: mdl-38528143
ABSTRACT
Osteoporosis (OP) is a prevalent global disease characterized by bone mass loss and microstructural destruction, resulting in increased bone fragility and fracture susceptibility. Our study aims to investigate the potential of kaempferol in preventing and treating OP through a combination of network pharmacology and molecular experiments. Kaempferol and OP-related targets were retrieved from the public database. A protein-protein interaction (PPI) network of common targets was constructed using the STRING database and visualized with Cytoscape 3.9.1 software. Enrichment analyses for GO and KEGG of potential therapeutic targets were conducted using the Hiplot platform. Molecular docking was performed using Molecular operating environment (MOE) software, and cell experiments were conducted to validate the mechanism of kaempferol in treating OP. Network pharmacology analysis identified 54 overlapping targets between kaempferol and OP, with 10 core targets identified. The primarily enriched pathways included atherosclerosis-related signaling pathways, the AGE/RAGE signaling pathway, and the TNF signaling pathway. Molecular docking results indicated stable binding of kaempferol and two target proteins, AKT1 and MMP9. In vitro cell experiments demonstrated significant upregulation of AKT1 expression in MC3T3-E1 cells (p < 0.001) with kaempferol treatment, along with downregulation of MMP9 expression (p < 0.05) compared to the control group. This study predicted the core targets and pathways of kaempferol in OP treatment using network pharmacology, and validated these findings through in vitro experiments, suggesting a promising avenue for future clinical treatment of OP.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osteoporose / Doenças Ósseas Metabólicas / Medicamentos de Ervas Chinesas Limite: Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osteoporose / Doenças Ósseas Metabólicas / Medicamentos de Ervas Chinesas Limite: Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article