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The cytotoxicity effect of bismuth oxide particles synthesized hydrothermally using different reaction temperatures in vitro.
Md Saad, W M; Mohd Noor, N A; Mohamad Salin, N S; Mohd, Z; Eshak, Z; Abdul Razak, H R.
Afiliação
  • Md Saad WM; Universiti Teknologi MARA, Faculty of Health Sciences, Centre of Medical Laboratory Technology, Puncak Alam Campus, Selangor, Malaysia. wanmaz755@uitm.edu.my.
  • Mohd Noor NA; Universiti Teknologi MARA, Faculty of Health Sciences, Centre of Medical Laboratory Technology, Puncak Alam Campus, Selangor, Malaysia.
  • Mohamad Salin NS; Universiti Teknologi MARA, Faculty of Health Sciences, Centre of Medical Laboratory Technology, Puncak Alam Campus, Selangor, Malaysia.
  • Mohd Z; Universiti Teknologi MARA, Faculty of Health Sciences, Centre of Medical Imaging, Puncak Alam Campus, Selangor, Malaysia.
  • Eshak Z; Universiti Teknologi MARA, Faculty of Pharmacy, Imaging Centre (IMACE), Puncak Alam Campus, Selangor, Malaysia.
  • Abdul Razak HR; University of Exeter, College of Medicine and Health, Medical Imaging Program, St. Luke's Campus, EX1 2LU, Devon, United Kingdom.
Med J Malaysia ; 79(Suppl 1): 8-13, 2024 Mar.
Article em En | MEDLINE | ID: mdl-38555879
ABSTRACT

INTRODUCTION:

Bismuth oxide (Bi2O3) particles gained attention in preclinical research especially in medical imaging. Bismuth oxide with its long circulation time is an alternative to the current iodine contrast media which directly possesses high X-ray attenuation coefficient. Exploration of bismuth compound is hampered owing to challenges in synthesizing control for in vivo stability. MATERIALS AND

METHODS:

This study aimed are to characterize Bi2O3 particles synthesized at 60, 90 and 120 °C via hydrothermal method and investigated cytotoxicity of cell viability assay, cell morphology analysis, intracellular reactive oxygen species (ROS) assay and expression of ER stress genes by real-time PCR.

RESULTS:

Results indicated that the size of rod-shaped Bi2O3 particles increased with rising synthesizing temperatures. The cytotoxicity of Bi2O3 particles in Chang liver cells was size-dependent. Bigger-sized Bi2O3 particles resulted in lesser toxicity effects. mRNA expressions of GRP78 and C/EBP homologous protein (CHOP) were down-regulated in all treated Chang liver cells due to the increasing size of Bi2O3 particles. Bi2O3 particles synthesized at 120 °C was found to be less toxic than iodine.

CONCLUSION:

Data suggested that the response of Chang liver cells to Bi2O3 particle cytotoxicity has a significant relationship with its reaction temperatures. This outcome is important in hazard assessment of Bi2O3 particles as a new contrast media and provides better understanding in synthesizing control to enhance its biocompatibility.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bismuto / Iodo Limite: Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bismuto / Iodo Limite: Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article