Droplet-based proteomics reveals CD36 as a marker for progenitors in mammary basal epithelium.
Cell Rep Methods
; 4(4): 100741, 2024 Apr 22.
Article
em En
| MEDLINE
| ID: mdl-38569541
ABSTRACT
Deep proteomic profiling of rare cell populations has been constrained by sample input requirements. Here, we present DROPPS (droplet-based one-pot preparation for proteomic samples), an accessible low-input platform that generates high-fidelity proteomic profiles of 100-2,500 cells. By applying DROPPS within the mammary epithelium, we elucidated the connection between mitochondrial activity and clonogenicity, identifying CD36 as a marker of progenitor capacity in the basal cell compartment. We anticipate that DROPPS will accelerate biology-driven proteomic research for a multitude of rare cell populations.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células-Tronco
/
Biomarcadores
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Antígenos CD36
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Proteômica
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Glândulas Mamárias Animais
Limite:
Animals
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Female
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Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article