In-vitro Clinical Diagnostics using RNA-Cleaving DNAzymes.
Chembiochem
; 25(11): e202400085, 2024 Jun 03.
Article
em En
| MEDLINE
| ID: mdl-38574237
ABSTRACT
Over the last three decades, significant advancements have been made in the development of biosensors and bioassays that use RNA-cleaving DNAzymes (RCDs) as molecular recognition elements. While early examples of RCDs were primarily responsive to metal ions, the past decade has seen numerous RCDs reported for more clinically relevant targets such as bacteria, cancer cells, small metabolites, and protein biomarkers. Over the past 5â
years several RCD-based biosensors have also been evaluated using either spiked biological matrixes or patient samples, including blood, serum, saliva, nasal mucus, sputum, urine, and faeces, which is a critical step toward regulatory approval and commercialization of such sensors. In this review, an overview of the methods used to generate RCDs and the properties of key RCDs that have been utilized for inâ
vitro testing is first provided. Examples of RCD-based assays and sensors that have been used to test either spiked biological samples or patient samples are then presented, highlighting assay performance in different biological matrixes. A summary of current prospects and challenges for development of inâ
vitro diagnostic tests incorporating RCDs and an overview of future directions of the field is also provided.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
/
DNA Catalítico
Limite:
Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article