Diagnostic value and cognitive regulatory roles of long non-coding RNA UCA1 in Alzheimer's disease.
Neurosci Lett
; 829: 137765, 2024 Apr 23.
Article
em En
| MEDLINE
| ID: mdl-38583504
ABSTRACT
BACKGROUND:
To explore the diagnostic role and potential mechanism of serum lncRNA UCA1 in Alzheimer's disease (AD).METHODS:
UCA1 concentration was determined using quantitative RT-PCR. The receiver operating characteristic curve was plotted to assess the diagnostic value. Cell viability and apoptotic capacity were assessed by cell counting kit-8 (CCK-8) and flow cytometry. Water maze experiments were used to test cognitive function in mice. The target genes of UCA1 were identified with a dual luciferase reporter assay. Functional and pathway analysis of miR-342-3p target genes was determined using enrichment analysis.RESULTS:
The concentration of UCA1 was elevated in the AD group and represented a diagnostic possibility of AD. The silenced UCA1 reduced the roles of Aß on viability and apoptosis of SHâSY5Y cells by sponging miR-342-3p. The impaired cognitive impairment was partly recovered by the knockdown of the UCA1/miR-342-3p axis. Potential targets of miR-342-3p were enriched in function and pathways related to AD progression.CONCLUSION:
The UCA1/miR-342-3p axis contributed to the occurrence of AD by regulating cognitive ability.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
MicroRNAs
/
Doença de Alzheimer
/
RNA Longo não Codificante
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article