Effect of Hypertonic Saline Solution on the Ventilatory Mechanics of Lungs Donated After Brain Death.
J Surg Res
; 298: 109-118, 2024 Jun.
Article
em En
| MEDLINE
| ID: mdl-38603941
ABSTRACT
INTRODUCTION:
Brain death (BD) compromises the viability of the lung for donation. Hypertonic saline solution (HSS) induces rapid intravascular volume expansion and immunomodulatory action. We investigated its role in ventilatory mechanics (VMs) and in the inflammatory activity of the lungs of rats subjected to BD.METHODS:
Wistar rats were divided into four groups control, n = 10 intact rats subjected to extraction of the heart-lung block; BD, n = 8 (BD) rats treated with isotonic saline solution (4 mL/kg) immediately after BD; hypertonic saline 0 h, n = 9 (Hip.0') rats treated with HSS (4 mL/kg) immediately after BD; and hypertonic saline 1 h, n = 9 (Hip.60'), rats treated with HSS (4 mL/kg) 60 min after BD. The hemodynamic characteristics, gas exchange, VMs, inflammatory mediators, and histopathological evaluation of the lung were evaluated over 240 min of BD.RESULTS:
In VMs, we observed increased airway resistance, tissue resistance, tissue elastance, and respiratory system compliance in the BD group (P < 0.037), while the treated groups showed no impairment over time (P > 0.05). In the histological analysis, the BD group showed a greater area of perivascular edema and a higher neutrophil count than the control group and the Hip.60' group (P < 0.05).CONCLUSIONS:
Treatment with HSS was effective in preventing changes in the elastic and resistive pulmonary components, keeping them at baseline levels. Late treatment reduced perivascular and neutrophilic edema in lung tissue.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Morte Encefálica
/
Ratos Wistar
/
Pulmão
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article