Massilia luteola sp. nov., a novel indole-producing and cellulose-degrading bacterium isolated from soil.
Int J Syst Evol Microbiol
; 74(4)2024 Apr.
Article
em En
| MEDLINE
| ID: mdl-38619981
ABSTRACT
A Gram-stain-negative, rod-shaped, indole-producing, and cellulose-degrading bacterial strain, designated NEAU-G-C5T, was isolated from soil collected from a forest in Dali city, Yunnan province, south China. 16S rRNA gene sequence analysis showed that strain NEAU-G-C5T was assigned to the genus Massilia and showed high sequence similarities to Massilia phosphatilytica 12-OD1T (98.32â%) and Massilia putida 6 NM-7T (98.41â%). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-G-C5T formed a lineage related to M. phosphatilytica 12-OD1T and M. putida 6 NM-7T. The major fatty acids of the strain were C16ââ0, C16ââ1 ω7c, and C17ââ0 cyclo. The respiratory quinone was Q-8. The polar lipid profile of the strain showed the presence of diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. In addition, the average nucleotide identity values between strain NEAU-G-C5T and its reference strains M. phosphatilytica 12-OD1T, M. putida 6 NM-7T, M. norwichensis NS9T, and M. kyonggiensis TSA1T were 89.7, 88.2, 81.3, and 88.0â%, respectively, and the levels of digital DNA-DNA hybridization between them were found to be 58.5â% (54.9-62.0â%), 53.2â% (49.8-56.7â%), 31.9â% (28.6-35.5â%), and 57.7â% (54.1-61.2â%), respectively, which were lower than the accepted threshold values of 95-96â% and 70â%, respectively. The DNA G+C content of strain NEAU-G-C5T was 66.5âmol%. The strain could produce indoleacetic acid and cellulase. On the basis of the phenotypic, genotypic, and chemotaxonomic characteristics, we conclude that strain NEAU-G-C5T represents a novel species of the genus Massilia, for which the name Massilia luteola sp. nov. is proposed. The type strain is NEAU-G-C5T (=MCCC 1K08668T=KCTC 8080T).
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01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fosfolipídeos
/
Ácidos Graxos
País/Região como assunto:
Asia
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article