Stabilization of galactose oxidase by high hydrostatic pressure: Insights on the role of cavities size.
Biotechnol Bioeng
; 121(7): 2057-2066, 2024 Jul.
Article
em En
| MEDLINE
| ID: mdl-38650386
ABSTRACT
High hydrostatic pressure stabilized galactose oxidase (GaOx) at 70.0-80.0°C against thermal inactivation. The pseudo-first-order rate constant of inactivation kinact decreased by a factor of 8 at 80°C and by a factor of 44 at 72.5°C. The most pronounced effect of pressure was at the lowest studied temperature of 70.0°C with an activation volume of inactivation ΔV of 78.8 cm3 mol-1. The optimal pressure against thermal inactivation was between 200 and 300 MPa. Unlike other enzymes, as temperature increased the ΔV of inactivation decreased, and as pressure increased the activation energy of inactivation Eai increased. Combining the results for GaOx with earlier research on the pressure-induced stabilization of other enzymes suggests that ΔV of inactivation correlates with the total molar volume of cavities larger than ~100 Å3 in enzyme monomers for enzymes near the optimal pH and whose thermal unfolding is not accompanied by oligomer dissociation.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Estabilidade Enzimática
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Galactose Oxidase
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Pressão Hidrostática
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article