Effect of Press-Fit Size on Insertion Mechanics and Cartilage Viability in Human and Ovine Osteochondral Grafts.
Cartilage
; : 19476035241247297, 2024 Apr 23.
Article
em En
| MEDLINE
| ID: mdl-38651510
ABSTRACT
OBJECTIVE:
The osteochondral allograft procedure uses grafts constructed larger than the recipient site to stabilize the graft, in what is known as the press-fit technique. This research aims to characterize the relationships between press-fit size, insertion forces, and cell viability in ovine and human osteochondral tissue.DESIGN:
Human (4 donors) and ovine (5 animals) articular joints were used to harvest osteochondral grafts (4.55 mm diameter, N = 33 Human, N = 35 Ovine) and create recipient sites with grafts constructed to achieve varying degrees of press fit (0.025-0.240 mm). Donor grafts were inserted into recipient sites while insertion forces were measured followed by quantification of chondrocyte viability and histological staining to evaluate the extracellular matrix.RESULTS:
Both human and ovine tissues exhibited similar mechanical and cellular responses to changes in press-fit. Insertion forces (Human 3-169 MPa, Ovine 36-314 MPa) and cell viability (Human 16%-89% live, Ovine 2%-76% live) were correlated to press-fit size for both human (force r = 0.539, viability r = -0.729) and ovine (force r = 0.655, viability r = -0.714) tissues. In both species, a press-fit above 0.14 mm resulted in reduced cell viability below a level acceptable for transplantation, increased insertion forces, and reduced linear correlation to press-fit size compared to samples with a press-fit below 0.14 mm.CONCLUSIONS:
Increasing press-fit size required increased insertion forces and resulted in reduced cell viability. Ovine and human osteochondral tissues responded similarly to impact insertion and varying press-fit size, providing evidence for the use of the ovine model in allograft-related research.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article