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Enhanced detection of ligand-PPARγ binding based on surface plasmon resonance through complexation with SRC1- or NCOR2-related polypeptide.
Wang, Yiting; Luo, Mingzhu; Che, Luyang; Wu, Qixin; Li, Jingzhe; Ma, Yanyan; Wang, Jingyi; Liu, Changzhen.
Afiliação
  • Wang Y; Experimental Research Center of China Academy of Chinese Medical Sciences, Beijing, China.
  • Luo M; Experimental Research Center of China Academy of Chinese Medical Sciences, Beijing, China.
  • Che L; Department of Vascular and Endovascular Surgery, People's Liberation Army General Hospital Hainan Hospital, Sanya, Hainan Province, China.
  • Wu Q; Experimental Research Center of China Academy of Chinese Medical Sciences, Beijing, China.
  • Li J; Experimental Research Center of China Academy of Chinese Medical Sciences, Beijing, China.
  • Ma Y; Experimental Research Center of China Academy of Chinese Medical Sciences, Beijing, China.
  • Wang J; Experimental Research Center of China Academy of Chinese Medical Sciences, Beijing, China.
  • Liu C; Experimental Research Center of China Academy of Chinese Medical Sciences, Beijing, China. Electronic address: lcz0220@163.com.
Int J Biol Macromol ; 268(Pt 1): 131865, 2024 May.
Article em En | MEDLINE | ID: mdl-38670200
ABSTRACT
A previous study reported the use of a biosensing technique based on surface plasmon resonance (SPR) for the ligand binding detection of peroxisome proliferator activator receptor gamma (PPARγ). This detection was designed based on the structural properties of PPARγ. Because of cross-linked protein inactivation and the low molecular weight of conventional ligands, direct ligand binding detection based on SPR has low stability and repeatability. In this study, we report an indirect response methodology based on SPR technology in which anti-His CM5 chip binds fresh PPARγ every cycle, resulting in more stable detection. We developed a remarkable improvement in ligand-protein binding detectability in vitro by introducing two coregulator-related polypeptides into this system. In parallel, a systematic indirect response methodology can reflect the interaction relationship between ligands and proteins to some extent by detecting the changes in SA-SRC1 and GST-NCOR2 binding to PPARγ. Rosiglitazone, a PPARγ agonist with strong affinity, is a potent insulin-sensitizing agent. Some ligands may be competitively exerted at the same sites of PPARγ (binding rosiglitazone). We demonstrated using indirect response methodology that selective PPARγ modulator (SPPARM) candidates of PPARγ can be found by competing for the binding of the rosiglitazone site on PPARγ, although they may have no effect on polypeptides and PPARγ binding.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ligação Proteica / Ressonância de Plasmônio de Superfície / PPAR gama / Coativador 1 de Receptor Nuclear Limite: Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ligação Proteica / Ressonância de Plasmônio de Superfície / PPAR gama / Coativador 1 de Receptor Nuclear Limite: Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article