Protocol for isolating amnion cells from human and non-human primate placenta for flow cytometry and transcriptomics.
STAR Protoc
; 5(2): 103044, 2024 Jun 21.
Article
em En
| MEDLINE
| ID: mdl-38678572
ABSTRACT
The amnion is a thin layer of fetal origin in contact with the amniotic fluid which plays a key role at the feto-maternal interface during pregnancy. Here, we present a protocol for isolation of human and Rhesusmacaque amnion cells. We describe steps for tissue dissection, cell isolation for flow cytometry analysis, and RNA isolation for RNA sequencing library preparation and analysis. This protocol can provide insights into altered immunological pathways during intrauterine infections to develop new therapeutic strategies. For complete details on the use and execution of this protocol, please refer to Presicce et al.1.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Placenta
/
Separação Celular
/
Citometria de Fluxo
/
Âmnio
Limite:
Animals
/
Female
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Humans
/
Pregnancy
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article