Effects of CDDO-EA in sepsis-induced acute lung injury: mouse model of endotoxaemia.
Wiad Lek
; 77(3): 497-505, 2024.
Article
em En
| MEDLINE
| ID: mdl-38691792
ABSTRACT
OBJECTIVE:
Aim:
The aim of this research is to clarify the potential effect of CDDO-EA against experimentally sepsis induced lung injury in mice. PATIENTS ANDMETHODS:
Materials andMethods:
Mice have divided into four groups Sham group CLP group, Vehicle-treatment group, CDDO-EA-treated group mice in this group received CDDO-EA 2mg/kg intraperitoneally, 1hr before CLP, then the animals were sacrificed 24hr after CLP. After exsAngpuinations, tissue samples of lung were collected, followed by markers measurement including, TNF-α, IL-1ß, VEGF, MPO, caspase11, Angp-1and Angp-2 by ELISA, gene expression of TIE2 and VE-cadherin by qRT-PCR, in addition to histopathological study.RESULTS:
Results:
A significant elevation (p<0.05) in TNF-α, IL-1ß, MPO, ANGP-2, VEGF, CASPASE 11 in CLP and vehicle groups when compared with sham group. CDDO-EA group showed significantly lower levels p<0.05, level of ANGP-1 was significantly lower p<0.05 in the CLP and vehicle groups as compared with the sham group. Quantitative real-time PCR demonstrated a significant decrement in mRNA expression of TIE2&ve-cadherin genes p<0.05 in sepsis & vehicle.CONCLUSION:
Conclusions:
CDDO-EA has lung protective effects due to its anti-inflammatory and antiAngpiogenic activity, additionally, CDDO-EA showes a lung protective effect as they affect tissue mRNA expression of TIE2 and cadherin gene. Furthermore, CDDO-EA attenuate the histopathological changes that occur during polymicrobial sepsis thereby lung protection effect.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sepse
/
Endotoxemia
/
Modelos Animais de Doenças
/
Lesão Pulmonar Aguda
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article