Marinomonas rhodophyticola sp. nov. and Marinomonas phaeophyticola sp. nov., isolated from marine algae.
Int J Syst Evol Microbiol
; 74(5)2024 May.
Article
em En
| MEDLINE
| ID: mdl-38700924
ABSTRACT
Two Gram-stain-negative, facultatively aerobic, and motile rod bacteria, designated as strains KJ51-3T and 15G1-11T, were isolated from marine algae collected in the Republic of Korea. Both strains exhibited catalase- and oxidase-positive activities. Optimum growth conditions for strain KJ51-3T were observed at 30â°C and pH 6.0-8.0, with 1.0-7.0â% (w/v) NaCl, whereas strain 15G1-11T exhibited optimal growth at 30â°C, pH 7.0, and 1.0-5.0â% NaCl. Major fatty acids detected in both strains included C16ââ0, C10ââ0 3-OH and summed features 3 (C16ââ1 ω7c and/or C16ââ1 ω6c) and 8 (C18ââ1 ω7c and/or C18ââ1 ω6c). As for polar lipids, strain KJ51-3T contained phosphatidylethanolamine (PE), phosphatidylglycerol (PG), diphosphatidylglycerol, and two unidentified phospholipids, whereas strain 15G1-11T had PE, PG, and an unidentified aminolipid. Ubiquinone-8 was the predominant respiratory quinone in both strains, with minor detection of ubiquinone-9 in strain KJ51-3T. The genomic DNA G+C contents were 44.0âmol% for strain KJ51-3T and 40.5âmol% for strain 15G1-11T. Phylogenetic analyses based on both 16S rRNA gene and genome sequences placed strains KJ51-3T and 15G1-11T into distinct lineages within the genus Marinomonas, most closely related to Marinomonas arctica 328T (98.6â%) and Marinomonas algicola SM1966T (98.3â%), respectively. Strains KJ51-3T and 15G1-11T exhibited a 94.6â% 16S rRNA gene sequence similarity and a 70.7â% average nucleotide identity (ANI), with ANI values of 91.9 and 79.3â% between them and M. arctica 328T and M. algicola SM1966T, respectively, indicating that they represent novel species. In summary, based on their phenotypic, chemotaxonomic, and phylogenetic properties, strains KJ51-3T and 15G1-11T are proposed to represent novel species within the genus Marinomonas, for which the names Marinomonas rhodophyticola sp. nov. (KJ51-3T=KACC 22756T=JCM 35591T) and Marinomonas phaeophyticola sp. nov. (15G1-11T=KACC 22593T=JCM 35412T) are respectively proposed.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fosfolipídeos
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Filogenia
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Composição de Bases
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DNA Bacteriano
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RNA Ribossômico 16S
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Técnicas de Tipagem Bacteriana
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Ubiquinona
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Análise de Sequência de DNA
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Marinomonas
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Ácidos Graxos
País/Região como assunto:
Asia
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article