A label-free ratiometric homogeneous electrochemical aptasensor based on dual catalytic hairpin self-assembly for rapid and sensitive detection of ochratoxin A in food.
Food Chem
; 453: 139651, 2024 Sep 30.
Article
em En
| MEDLINE
| ID: mdl-38761736
ABSTRACT
The food contamination with Ochratoxin A (OTA) has highlighted the need to create precise, sensitive, and convenient techniques. Herein, we proposed a label-free and immobilization-free ratiometric homogeneous electrochemical aptasensor based on dual catalytic hairpin self-assembly (CHA) for OTA detection. Methylene blue (MB) and ferrocene (Fc) in solution were utilized as label-free signaling molecules, generating a response signal (IMB) and a reference signal (IFc), respectively. The ratio of IMB/IFc was utilized as a measure to quantify OTA. Dual CHA was exploited to increase the ratiometric signal and enhance the amplification efficiency. This aptasensor achieved trace-level detection for OTA over a linear range of lower concentrations (1.0 × 10-3 ng/mL-1.0 × 103 ng/mL) with LOD of 92 fg/mL. The aptasensor was successfully applied to detect OTA in cereal and wine, with comparable results of HPLC-MS/MS. This strategy provided a viable platform for rapid, sensitive, and accurate detection of OTA in food.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vinho
/
Técnicas Biossensoriais
/
Contaminação de Alimentos
/
Aptâmeros de Nucleotídeos
/
Técnicas Eletroquímicas
/
Limite de Detecção
/
Ocratoxinas
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article