Protocol for separation of fungal extracellular vesicles using ultracentrifugation from solid medium cultures.
STAR Protoc
; 5(2): 103069, 2024 Jun 21.
Article
em En
| MEDLINE
| ID: mdl-38771694
ABSTRACT
Extracellular vesicles (EVs) have been identified in diverse fungi, including human pathogens. In this protocol, we present two techniques for isolating and analyzing fungal EVs. The first is for high-throughput screening, and the second is for yielding concentrated samples suitable for centrifugation-based density gradients. We describe steps for analytical assays such as nano-flow cytometry and nanoparticle tracking analysis to measure EV dimensions and concentration. EV suspensions can serve diverse assays, including electron microscopy, compositional determination, and cell-to-cell communication assays. For complete details on the use and execution of this protocol, please refer to Rizzo et al.,1 Rizzo et al.,2 Reis et al.,3 and Reis et al.4.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ultracentrifugação
/
Vesículas Extracelulares
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Fungos
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article