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Effect of hypercholesterolemia on circulating and cardiomyocyte-derived extracellular vesicles.
Kovácsházi, Csenger; Hambalkó, Szabolcs; Sayour, Nabil V; Gergely, Tamás G; Brenner, Gábor B; Pelyhe, Csilla; Kapui, Dóra; Weber, Bennet Y; Hültenschmidt, Alexander L; Pállinger, Éva; Buzás, Edit I; Zolcsák, Ádám; Kiss, Bálint; Bozó, Tamás; Csányi, Csilla; Kósa, Nikolett; Kellermayer, Miklós; Farkas, Róbert; Karvaly, Gellért B; Wynne, Kieran; Matallanas, David; Ferdinandy, Péter; Giricz, Zoltán.
Afiliação
  • Kovácsházi C; Department of Pharmacology and Pharmacotherapy, Semmelweis University, Budapest, Hungary.
  • Hambalkó S; Department of Pharmacology and Pharmacotherapy, Semmelweis University, Budapest, Hungary.
  • Sayour NV; Department of Pharmacology and Pharmacotherapy, Semmelweis University, Budapest, Hungary.
  • Gergely TG; Department of Pharmacology and Pharmacotherapy, Semmelweis University, Budapest, Hungary.
  • Brenner GB; Department of Pharmacology and Pharmacotherapy, Semmelweis University, Budapest, Hungary.
  • Pelyhe C; Department of Pharmacology and Pharmacotherapy, Semmelweis University, Budapest, Hungary.
  • Kapui D; Department of Pharmacology and Pharmacotherapy, Semmelweis University, Budapest, Hungary.
  • Weber BY; Department of Pharmacology and Pharmacotherapy, Semmelweis University, Budapest, Hungary.
  • Hültenschmidt AL; Department of Pharmacology and Pharmacotherapy, Semmelweis University, Budapest, Hungary.
  • Pállinger É; Institute of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary.
  • Buzás EI; Institute of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary.
  • Zolcsák Á; ELKH-SE Translational Extracellular Vesicle Research Group, Budapest, Hungary.
  • Kiss B; HCEMM-SU Extracellular Vesicle Research Group, Budapest, Hungary.
  • Bozó T; Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary.
  • Csányi C; Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary.
  • Kósa N; HUNREN-SE Biophysical Virology Research Group, Budapest, Hungary.
  • Kellermayer M; Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary.
  • Farkas R; Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary.
  • Karvaly GB; Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary.
  • Wynne K; Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary.
  • Matallanas D; HUNREN-SE Biophysical Virology Research Group, Budapest, Hungary.
  • Ferdinandy P; Department of Laboratory Medicine, Laboratory of Mass Spectrometry and Separation Technology, Semmelweis University, Budapest, Hungary.
  • Giricz Z; Department of Laboratory Medicine, Laboratory of Mass Spectrometry and Separation Technology, Semmelweis University, Budapest, Hungary.
Sci Rep ; 14(1): 12016, 2024 05 26.
Article em En | MEDLINE | ID: mdl-38797778
ABSTRACT
Hypercholesterolemia (HC) induces, propagates and exacerbates cardiovascular diseases via various mechanisms that are yet not properly understood. Extracellular vesicles (EVs) are involved in the pathomechanism of these diseases. To understand how circulating or cardiac-derived EVs could affect myocardial functions, we analyzed the metabolomic profile of circulating EVs, and we performed an in-depth analysis of cardiomyocyte (CM)-derived EVs in HC. Circulating EVs were isolated with Vezics technology from male Wistar rats fed with high-cholesterol or control chow. AC16 human CMs were treated with Remembrane HC supplement and EVs were isolated from cell culture supernatant. The biophysical properties and the protein composition of CM EVs were analyzed. THP1-ASC-GFP cells were treated with CM EVs, and monocyte activation was measured. HC diet reduced the amount of certain phosphatidylcholines in circulating EVs, independently of their plasma level. HC treatment significantly increased EV secretion of CMs and greatly modified CM EV proteome, enriching several proteins involved in tissue remodeling. Regardless of the treatment, CM EVs did not induce the activation of THP1 monocytes. In conclusion, HC strongly affects the metabolome of circulating EVs and dysregulates CM EVs, which might contribute to HC-induced cardiac derangements.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ratos Wistar / Miócitos Cardíacos / Vesículas Extracelulares / Hipercolesterolemia Limite: Animals / Humans / Male Idioma: En Ano de publicação: 2024 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ratos Wistar / Miócitos Cardíacos / Vesículas Extracelulares / Hipercolesterolemia Limite: Animals / Humans / Male Idioma: En Ano de publicação: 2024 Tipo de documento: Article