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Temozolomide alleviates breast carcinoma via the inhibition of EGFR/ERK/ MMP-1 pathway with induction of apoptotic events.
Zhu, Weijun; Zhang, Fengjun; Wang, Maoyun; Meng, Shuai; Ren, Fang.
Afiliação
  • Zhu W; Taizhou Municipal Hospital - Department of Pathology - Zhejiang Province, Taizhou Zhejiang, China.
  • Zhang F; The 940th Hospital of Joint Logistics Support Force of PLA - Department of Mammary Gland - Lanzhou, Gansu, China.
  • Wang M; First Medical Center of PLA General Hospital - Department of Traditional Chinese Medicine - Beijing, China.
  • Meng S; First Medical Center of PLA General Hospital - Department of Traditional Chinese Medicine - Beijing, China.
  • Ren F; First Medical Center of PLA General Hospital - Department of Traditional Chinese Medicine - Beijing, China.
Acta Cir Bras ; 39: e391624, 2024.
Article em En | MEDLINE | ID: mdl-38808816
ABSTRACT

PURPOSE:

To evaluate the chemotherapeutic activity of temozolomide counter to mammary carcinoma.

METHODS:

In-vitro anticancer activity has been conducted on MCF7 cells, and mammary carcinoma has been induced in Wistar rats by introduction of 7, 12-Dimethylbenz(a)anthracene (DMBA), which was sustained for 24 weeks. Histopathology, immunohistochemistry, cell proliferation study and apoptosis assay via TUNEL method was conducted to evaluate an antineoplastic activity of temozolomide in rat breast tissue.

RESULTS:

IC50 value of temozolomide in MCF7 cell has been obtained as 103 µM, which demonstrated an initiation of apoptosis. The temozolomide treatment facilitated cell cycle arrest in G2/M and S phase dose dependently. The treatment with temozolomide suggested decrease of the hyperplastic abrasions and renovation of the typical histological features of mammary tissue. Moreover, temozolomide therapy caused the downregulation of epidermal growth factor receptor, extracellular signal-regulated kinase, and metalloproteinase-1 expression and upstream of p53 and caspase-3 proliferation to indicate an initiation of apoptotic events.

CONCLUSIONS:

The occurrence of mammary carcinoma has been significantly decreased by activation of apoptotic pathway and abrogation of cellular propagation that allowable for developing a suitable mechanistic pathway of temozolomide in order to facilitate chemotherapeutic approach.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ratos Wistar / Apoptose / Antineoplásicos Alquilantes / Receptores ErbB / Temozolomida Limite: Animals / Female / Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ratos Wistar / Apoptose / Antineoplásicos Alquilantes / Receptores ErbB / Temozolomida Limite: Animals / Female / Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article