SLC7A11-mediated cystine import protects against NDUFS7 deficiency-induced cell death in HEK293T cells.
Biochem Biophys Res Commun
; 723: 150178, 2024 Sep 03.
Article
em En
| MEDLINE
| ID: mdl-38823363
ABSTRACT
Cell models of mitochondrial complex â
(Câ
) deficiency display significant elevations in reactive oxygen species (ROS) levels and an increase in cellular apoptosis. However, the underlying mechanisms governing anti-apoptotic processes in Câ
-deficient cells remain elusive. Here, we introduced a mutation in NDUFS7, a crucial subunit of CI, in HEK293T cells and found that the absence of NDUFS7 resulted in reduced cell proliferation, elevated cell death, and increased susceptibility to oxidative stress. Mechanismly, we revealed that the upregulation of SLC7A11 played a crucial role in mitigating cell death resulting from NDUFS7 deficiency. Specifically, the increased expression of SLC7A11 enhanced cystine import, which subsequently reduced cell death by promoting the biosynthesis of reduced glutathione (GSH). Collectively, our findings suggest that SLC7A11-mediated cystine import, representing a novel pathway independent of NADPH production, plays a vital role in protection against NDUFS7 deficiency-induced cell death. This novel pathway provides potential insights into the understanding of pathogenic mechanisms and the therapeutic management of mitochondrial disorders associated with Câ
deficiency.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cistina
/
Sistema y/ de Transporte de Aminoácidos
/
Complexo I de Transporte de Elétrons
Limite:
Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article