TSWIFT, a novel method for iterative staining of embedded and mounted human brain sections.
Sci Rep
; 14(1): 12688, 2024 06 03.
Article
em En
| MEDLINE
| ID: mdl-38830987
ABSTRACT
Comprehensive characterization of protein networks in mounted brain tissue represents a major challenge in brain and neurodegenerative disease research. In this study, we develop a simple staining method, called TSWIFT, to iteratively stain pre-mounted formalin fixed, paraffin embedded (FFPE) brain sections, thus enabling high-dimensional sample phenotyping. We show that TSWIFT conserves tissue architecture and allows for relabeling a single mounted FFPE sample more than 10 times, even after prolonged storage at 4 °C. Our results establish TSWIFT as an efficient method to obtain integrated high-dimensional knowledge of cellular proteomes by analyzing mounted FFPE human brain tissue.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Coloração e Rotulagem
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Encéfalo
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Inclusão em Parafina
Limite:
Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article