The omega-3 postbiotic trans-10-cis-15-octadecadienoic acid attenuates contact hypersensitivity in mice through downregulation of vascular endothelial growth factor A.
Front Cell Infect Microbiol
; 14: 1355679, 2024.
Article
em En
| MEDLINE
| ID: mdl-38841110
ABSTRACT
Intestinal bacteria metabolize dietary substances to produce bioactive postbiotics, among which some are recognized for their role in promoting host health. We here explored the postbiotic potential of two omega-3 α-linolenic acid-derived metabolites trans-10-cis-15-octadecadienoic acid (t10,c15-182) and cis-9-cis-15-octadecadienoic acid (c9,c15-182). Dietary intake of lipids rich in omega-3 α-linolenic acid elevated levels of t10,c15-182 and c9,c15-182 in the serum and feces of mice, an effect dependent on the presence of intestinal bacteria. Notably, t10,c15-182 mitigated skin inflammation in mice that became hypersensitive after exposure to 2,4-dinitrofluorobenzene, an experimental model for allergic contact dermatitis. In particular, t10,c15-182-but not c9,c15-182-attenuated ear swelling and edema, characteristic symptoms of contact hypersensitivity. The anti-inflammatory effects of t10,c15-182 were due to its ability to suppress the release of vascular endothelial growth factor A from keratinocytes, thereby mitigating the enhanced vascular permeability induced by hapten stimulation. Our study identified retinoid X receptor as a functional receptor that mediates the downregulation of skin inflammation upon treatment with t10,c15-182. Our results suggest that t10,c15-182 holds promise as an omega-3 fatty acid-derived postbiotic with potential therapeutic implications for alleviating the skin edema seen in allergic contact dermatitis-induced inflammation.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Regulação para Baixo
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Ácidos Graxos Ômega-3
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Fator A de Crescimento do Endotélio Vascular
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Modelos Animais de Doenças
Limite:
Animals
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Female
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Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article