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Single-cell and spatially resolved interactomics of tooth-associated keratinocytes in periodontitis.
Easter, Quinn T; Fernandes Matuck, Bruno; Beldorati Stark, Germán; Worth, Catherine L; Predeus, Alexander V; Fremin, Brayon; Huynh, Khoa; Ranganathan, Vaishnavi; Ren, Zhi; Pereira, Diana; Rupp, Brittany T; Weaver, Theresa; Miller, Kathryn; Perez, Paola; Hasuike, Akira; Chen, Zhaoxu; Bush, Mandy; Qu, Xufeng; Lee, Janice; Randell, Scott H; Wallet, Shannon M; Sequeira, Inês; Koo, Hyun; Tyc, Katarzyna M; Liu, Jinze; Ko, Kang I; Teichmann, Sarah A; Byrd, Kevin M.
Afiliação
  • Easter QT; Lab of Oral & Craniofacial Innovation (LOCI), Department of Innovation & Technology Research, ADA Science & Research Institute, Gaithersburg, MD, USA.
  • Fernandes Matuck B; Lab of Oral & Craniofacial Innovation (LOCI), Department of Innovation & Technology Research, ADA Science & Research Institute, Gaithersburg, MD, USA.
  • Beldorati Stark G; Parse Biosciences, Seattle, WA, USA.
  • Worth CL; The Bioinformatics CRO, Orlando, FL, USA.
  • Predeus AV; Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK.
  • Fremin B; The Bioinformatics CRO, Orlando, FL, USA.
  • Huynh K; Department of Biostatistics, Virginia Commonwealth University, Richmond, VA, USA.
  • Ranganathan V; School of Computer Science, Carnegie Mellon University, Pittsburgh, PA, USA.
  • Ren Z; Biofilm Research Laboratories, Center for Innovation & Precision Dentistry, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA, USA.
  • Pereira D; Center for Oral Immunobiology and Regenerative Medicine, Barts Centre for Squamous Cancer, Institute of Dentistry, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, UK.
  • Rupp BT; Lab of Oral & Craniofacial Innovation (LOCI), Department of Innovation & Technology Research, ADA Science & Research Institute, Gaithersburg, MD, USA.
  • Weaver T; Lab of Oral & Craniofacial Innovation (LOCI), Department of Innovation & Technology Research, ADA Science & Research Institute, Gaithersburg, MD, USA.
  • Miller K; Akoya Biosciences Inc., Marlborough, MA, USA.
  • Perez P; Salivary Disorders Unit, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA.
  • Hasuike A; Lab of Oral & Craniofacial Innovation (LOCI), Department of Innovation & Technology Research, ADA Science & Research Institute, Gaithersburg, MD, USA.
  • Chen Z; Department of Periodontology, Nihon University School of Dentistry, Tokyo, Japan.
  • Bush M; Department of Periodontics, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA, USA.
  • Qu X; Respiratory TRACTS Core, Marsico Lung Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
  • Lee J; VCU Massey Comprehensive Cancer Center, Bioinformatics Shared Resource Core, Virginia Commonwealth University, Richmond, VA, USA.
  • Randell SH; Craniofacial Anomalies & Regeneration Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA.
  • Wallet SM; Marsico Lung Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
  • Sequeira I; Division of Oral and Craniofacial Health Sciences, Adams School of Dentistry, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
  • Koo H; Department of Oral Biology, College of Dentistry, University of Florida, Gainesville, FL, USA.
  • Tyc KM; Center for Oral Immunobiology and Regenerative Medicine, Barts Centre for Squamous Cancer, Institute of Dentistry, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, UK.
  • Liu J; Biofilm Research Laboratories, Center for Innovation & Precision Dentistry, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA, USA.
  • Ko KI; Department of Biostatistics, Virginia Commonwealth University, Richmond, VA, USA.
  • Teichmann SA; VCU Massey Comprehensive Cancer Center, Bioinformatics Shared Resource Core, Virginia Commonwealth University, Richmond, VA, USA.
  • Byrd KM; Department of Biostatistics, Virginia Commonwealth University, Richmond, VA, USA.
Nat Commun ; 15(1): 5016, 2024 Jun 14.
Article em En | MEDLINE | ID: mdl-38876998
ABSTRACT
Periodontitis affects billions of people worldwide. To address relationships of periodontal niche cell types and microbes in periodontitis, we generated an integrated single-cell RNA sequencing (scRNAseq) atlas of human periodontium (34-sample, 105918-cell), including sulcular and junctional keratinocytes (SK/JKs). SK/JKs displayed altered differentiation states and were enriched for effector cytokines in periodontitis. Single-cell metagenomics revealed 37 bacterial species with cell-specific tropism. Fluorescence in situ hybridization detected intracellular 16 S and mRNA signals of multiple species and correlated with SK/JK proinflammatory phenotypes in situ. Cell-cell communication analysis predicted keratinocyte-specific innate and adaptive immune interactions. Highly multiplexed immunofluorescence (33-antibody) revealed peri-epithelial immune foci, with innate cells often spatially constrained around JKs. Spatial phenotyping revealed immunosuppressed JK-microniches and SK-localized tertiary lymphoid structures in periodontitis. Here, we demonstrate impacts on and predicted interactomics of SK and JK cells in health and periodontitis, which requires further investigation to support precision periodontal interventions in states of chronic inflammation.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Periodontite / Queratinócitos / Comunicação Celular / Análise de Célula Única Limite: Adult / Female / Humans / Male Idioma: En Ano de publicação: 2024 Tipo de documento: Article
Texto completo
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PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Periodontite / Queratinócitos / Comunicação Celular / Análise de Célula Única Limite: Adult / Female / Humans / Male Idioma: En Ano de publicação: 2024 Tipo de documento: Article