Protocol to study the immune profile of syngeneic mouse tumor models.
STAR Protoc
; 5(3): 103139, 2024 Sep 20.
Article
em En
| MEDLINE
| ID: mdl-38878286
ABSTRACT
Flow cytometry, single-cell RNA sequencing, and other analyses enable us to capture immune profiles of the tumor microenvironment. Here, we present a protocol to characterize the immune profile of tumor-bearing mice. We describe steps for establishing mouse models and preparing single-cell suspensions from tumor tissue and other immune-related organs, which can be further analyzed by flow cytometry and other omics assays. We then detail procedures for staining, flow cytometry analysis, and phenotyping of the immune cell populations. For complete details on the use and execution of this protocol, please refer to Miyauchi et al.1.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Microambiente Tumoral
/
Citometria de Fluxo
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article