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Human Gonads Do Not Contribute to the Circulating Pool of 11-Oxygenated Androgens.
Charoensri, Suranut; Rege, Juilee; Lee, Chaelin; Marko, Xhorlina; Sherk, William; Sholinyan, Julieta; Rainey, William E; Turcu, Adina F.
Afiliação
  • Charoensri S; Division of Metabolism, Endocrinology and Diabetes, Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan 48109.
  • Rege J; Division of Endocrinology and Metabolism, Department of Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40000, Thailand.
  • Lee C; Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, Michigan 48109.
  • Marko X; Division of Metabolism, Endocrinology and Diabetes, Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan 48109.
  • Sherk W; Division of Vascular and Interventional Radiology, University of Michigan, Ann Arbor, Michigan 48109.
  • Sholinyan J; Division of Vascular and Interventional Radiology, University of Michigan, Ann Arbor, Michigan 48109.
  • Rainey WE; Division of Metabolism, Endocrinology and Diabetes, Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan 48109.
  • Turcu AF; Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, Michigan 48109.
Article em En | MEDLINE | ID: mdl-38885296
ABSTRACT
CONTEXT Androstenedione (A4) and testosterone (T) are produced by both the adrenal glands and the gonads. The adrenal enzyme 11ß-hydroxylase (CYP11B1) executes the final step in cortisol synthesis; CYP11B1 also uses A4 and T as substrates, generating 11-hydroxyandrostenedione and 11-hydroxytestosterone, respectively. It has been suggested that CYP11B1 is expressed in the gonads, yet the circulating levels of all 11-oxygenated androgens (11-oxyandrogens) are similar in males and females of reproductive ages, despite enormous differences in T.

OBJECTIVE:

To assess the gonadal contribution to the circulating pool of 11-oxyandrogens.

METHODS:

We used liquid chromatography-tandem mass spectrometry to measure 13 steroids, including traditional and 11-oxyandrogens in (I) paired gonadal and peripheral vein blood samples obtained during gonadal venograms from 11 patients (7 women), median age 37 (range 31-51 years); and (II) 17 women, median age 57 (range 41-81 years) before and after bilateral salpingo-oophorectomy (BSO). We also compared CYP11B1, 17α-hydroxylase/17,20-lyase (CYP17A1), and 3ß-hydroxysteroid dehydrogenase type 2 (HSD3B2) mRNA expression in adrenal, ovarian, and testicular tissue.

RESULTS:

A4, T, estradiol, estrone, progesterone, 17α- and 16α-hydroxyprogesterone were all higher in gonadal veins vs. periphery (p < 0.05 for all), while four 11-oxyandrogens were similar between matched gonadal and peripheral vein samples. Equally, in women who underwent BSO, A4 (median [interquartile range] 59.7 [47.7-67.6] ng/dL vs. 32.7 [27.4-47.8] ng/dL, p < 0.001) and T (24.1 [16.4-32.3] vs.15.5 [13.7-19.0] ng/dL, p < 0.001) declined, while 11-oxyandrogens remained stable. Gonadal tissue displayed negligible CYP11B1 mRNA.

CONCLUSION:

Despite producing substantial amounts of A4 and T, human gonads are not relevant sources of 11-oxyandrogens.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2024 Tipo de documento: Article