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Advanced optical assessment and modeling of extrusion bioprinting.
Lamberger, Zan; Schubert, Dirk W; Buechner, Margitta; Cabezas, Nathaly Chicaiza; Schrüfer, Stefan; Murenu, Nicoletta; Schaefer, Natascha; Lang, Gregor.
Afiliação
  • Lamberger Z; Department for Functional Materials in Medicine and Dentistry, University Hospital of Würzburg, 97070, Würzburg, Germany.
  • Schubert DW; Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058, Erlangen, Germany.
  • Buechner M; Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058, Erlangen, Germany.
  • Cabezas NC; Department for Functional Materials in Medicine and Dentistry, University Hospital of Würzburg, 97070, Würzburg, Germany.
  • Schrüfer S; Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058, Erlangen, Germany.
  • Murenu N; Institute for Clinical Neurobiology, University Hospital of Würzburg, 97078, Würzburg, Germany.
  • Schaefer N; Institute for Clinical Neurobiology, University Hospital of Würzburg, 97078, Würzburg, Germany.
  • Lang G; Department for Functional Materials in Medicine and Dentistry, University Hospital of Würzburg, 97070, Würzburg, Germany. gregor.lang@uni-wuerzburg.de.
Sci Rep ; 14(1): 13972, 2024 06 17.
Article em En | MEDLINE | ID: mdl-38886452
ABSTRACT
In the context of tissue engineering, biofabrication techniques are employed to process cells in hydrogel-based matrices, known as bioinks, into complex 3D structures. The aim is the production of functional tissue models or even entire organs. The regenerative production of biological tissues adheres to a multitude of criteria that ultimately determine the maturation of a functional tissue. These criteria are of biological nature, such as the biomimetic spatial positioning of different cell types within a physiologically and mechanically suitable matrix, which enables tissue maturation. Furthermore, the processing, a combination of technical procedures and biological materials, has proven highly challenging since cells are sensitive to stress, for example from shear and tensile forces, which may affect their vitality. On the other hand, high resolutions are pursued to create optimal conditions for subsequent tissue maturation. From an analytical perspective, it is prudent to first investigate the printing behavior of bioinks before undertaking complex biological tests. According to our findings, conventional shear rheological tests are insufficient to fully characterize the printing behavior of a bioink. For this reason, we have developed optical methods that, complementarily to the already developed tests, allow for quantification of printing quality and further viscoelastic modeling of bioinks.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Hidrogéis / Engenharia Tecidual / Bioimpressão / Impressão Tridimensional Limite: Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Hidrogéis / Engenharia Tecidual / Bioimpressão / Impressão Tridimensional Limite: Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article