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Nascent transcription reveals regulatory changes in extremophile fishes inhabiting hydrogen sulfide-rich environments.
Perry, Blair W; McGowan, Kerry L; Arias-Rodriguez, Lenin; Duttke, Sascha H; Tobler, Michael; Kelley, Joanna L.
Afiliação
  • Perry BW; School of Biological Sciences, Washington State University, Pullman, WA 99164, USA.
  • McGowan KL; School of Biological Sciences, Washington State University, Pullman, WA 99164, USA.
  • Arias-Rodriguez L; División Académica de Ciencias Biológicas, Universidad Juárez Autónoma de Tabasco, Villahermosa, Tabasco 86150, México.
  • Duttke SH; School of Molecular Biosciences, Washington State University, Pullman, WA 99164, USA.
  • Tobler M; Department of Biology, University of Missouri-St Louis, St Louis, MO 63121, USA.
  • Kelley JL; Whitney R. Harris World Ecology Center, University of Missouri-St Louis, St Louis, MO 63121, USA.
Proc Biol Sci ; 291(2025): 20240412, 2024 Jun.
Article em En | MEDLINE | ID: mdl-38889788
ABSTRACT
Regulating transcription allows organisms to respond to their environment, both within a single generation (plasticity) and across generations (adaptation). We examined transcriptional differences in gill tissues of fishes in the Poecilia mexicana species complex (family Poeciliidae), which have colonized toxic springs rich in hydrogen sulfide (H2S) in southern Mexico. There are gene expression differences between sulfidic and non-sulfidic populations, yet regulatory mechanisms mediating this gene expression variation remain poorly studied. We combined capped-small RNA sequencing (csRNA-seq), which captures actively transcribed (i.e. nascent) transcripts, and messenger RNA sequencing (mRNA-seq) to examine how variation in transcription, enhancer activity, and associated transcription factor binding sites may facilitate adaptation to extreme environments. csRNA-seq revealed thousands of differentially initiated transcripts between sulfidic and non-sulfidic populations, many of which are involved in H2S detoxification and response. Analyses of transcription factor binding sites in promoter and putative enhancer csRNA-seq peaks identified a suite of transcription factors likely involved in regulating H2S-specific shifts in gene expression, including several key transcription factors known to respond to hypoxia. Our findings uncover a complex interplay of regulatory processes that reflect the divergence of extremophile populations of P. mexicana from their non-sulfidic ancestors and suggest shared responses among evolutionarily independent lineages.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Poecilia / Sulfeto de Hidrogênio Limite: Animals País/Região como assunto: Mexico Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Poecilia / Sulfeto de Hidrogênio Limite: Animals País/Região como assunto: Mexico Idioma: En Ano de publicação: 2024 Tipo de documento: Article