A monoclonal antibody based solid-phase enzyme-binding assay to measure levels of placental alkaline phosphatase in serum of women during pregnancy.
J Immunol Methods
; 79(1): 99-108, 1985 May 10.
Article
em En
| MEDLINE
| ID: mdl-3889164
ABSTRACT
Monoclonal antibodies raised against highly purified placental alkaline phosphatase (PALP) reacted specifically with PALP and were found not to cross-react with the liver, kidney and intestinal enzymes. One of these antibodies (AP-3) was selected to develop an enzyme-binding assay to measure levels of PALP in the sera of 11 women throughout pregnancy. PALP activity rose sharply after 28 weeks of gestation reaching a peak at delivery followed by an immediate drop within 4 days to values approaching non-pregnant levels. The potential of using this assay as an index of placental function to monitor progress of a pregnancy is discussed.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Placenta
/
Técnicas Imunoenzimáticas
/
Fosfatase Alcalina
Limite:
Female
/
Humans
/
Pregnancy
Idioma:
En
Ano de publicação:
1985
Tipo de documento:
Article