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Synergic effect of combined xenogeneic mesenchymal stem cells and ceftriaxone on acute septic arthritis.
Sung, Pei-Hsun; Yin, Tsung-Cheng; Chiang, John Y; Chen, Chih-Hung; Huang, Chi-Ruei; Lee, Mel S; Yip, Hon-Kan.
Afiliação
  • Sung PH; Division of Cardiology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 833401, Taiwan, ROC.
  • Yin TC; Center for Shockwave Medicine and Tissue Engineering, Kaohsiung Chang Gung Memorial Hospital Kaohsiung, Kaohsiung 833401, Taiwan, ROC.
  • Chiang JY; Institute for Translational Research in Biomedicine, Kaohsiung Chang Gung Memorial Hospital Kaohsiung 833401, Taiwan, ROC.
  • Chen CH; Department of Orthopedics, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, 833401 Kaohsiung, Taiwan, ROC.
  • Huang CR; Center for General Education, Cheng Shiu University, Kaohsiung 833301, Taiwan, ROC.
  • Lee MS; Department of Computer Science and Engineering, National Sun Yat-Sen University, Kaohsiung 804201, Taiwan, ROC.
  • Yip HK; Department of Healthcare Administration and Medical Informatics, Kaohsiung Medical University, Kaohsiung 807378, Taiwan, ROC.
Stem Cells Transl Med ; 13(8): 724-737, 2024 Aug 16.
Article em En | MEDLINE | ID: mdl-38894649
ABSTRACT

BACKGROUND:

This study tested the hypothesis that combined ceftriaxone (Cef) and human umbilical cord-derived mesenchymal stem cells (HUCDMSCs) was better than either therapy for alleviating acute septic arthritis (ASA). METHODS AND

RESULTS:

Adult-male C57BL/6 mice were categorized into control group (Clt), group A (ASA only), group B [ASA + Cef (5 mg/kg, IM per day, at days 2 to 16 after ASA induction)], group C [ASA + HUCDMSCs (5 × 105 per mice at days 2, 3, 4 after ASA induction)], and group D (ASA + Cef + HUCDMSCs). Animals were euthanized by day 28. The result demonstrated that the body weight was significantly lower, whereas the ratio of kidney or spleen weight to WB, circulatory WBC count, bacterial colony-formation-unit from circulatory/kidney extraction were significantly higher in group A than in other groups (all P < .001). The proinflammatory cytokines (IL-6/TNF-α) of knee joint fluid were lowest in Clt and significantly and progressively reduced from groups A to D, whereas the circulatory levels of these 2 parameters at the time points of days 3/7/28 exhibited an identical pattern as knee joint fluid among the groups (all P-value < .0001). The scores of vertebral-bone destructions/inflamed synovium were lowest in Clt, highest in group A, significantly higher in group C than in groups B/D, and significantly higher in group C than in group D (all P < .0001).

CONCLUSION:

Combined antibiotics and Cef and HUCDMSCs was superior to just one therapy for suppressing circulatory and tissue levels of inflammation and knee joint destruction in ASA.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ceftriaxona / Artrite Infecciosa / Transplante de Células-Tronco Mesenquimais / Células-Tronco Mesenquimais / Camundongos Endogâmicos C57BL Limite: Animals / Humans / Male Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ceftriaxona / Artrite Infecciosa / Transplante de Células-Tronco Mesenquimais / Células-Tronco Mesenquimais / Camundongos Endogâmicos C57BL Limite: Animals / Humans / Male Idioma: En Ano de publicação: 2024 Tipo de documento: Article