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Red fermented rice elution fractions inhibits cancer cell proliferation by regulating the FGFR1/PI3K/AKT signaling pathway.
Guo, Zhongyuan; Zhao, Bingli; Song, Yafang; Yan, Wen; Xue, Lan; Liu, Xi; Wang, Zhimin; Pei, Huan; Yang, Hong.
Afiliação
  • Guo Z; College of Medicine, Henan University of Chinese Medicine, Henan Zhengzhou 450046, China; Institue of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
  • Zhao B; Yanjing Medical College,Capital Medical University, Beijing 101300, China.
  • Song Y; Yanjing Medical College,Capital Medical University, Beijing 101300, China.
  • Yan W; Yanjing Medical College,Capital Medical University, Beijing 101300, China.
  • Xue L; Beijing Peking University WBL Biotech Co., Ltd., Beijing 100094, China.
  • Liu X; Beijing Peking University WBL Biotech Co., Ltd., Beijing 100094, China.
  • Wang Z; Institue of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
  • Pei H; Beijing Peking University WBL Biotech Co., Ltd., Beijing 100094, China. Electronic address: peihuan@luye.com.
  • Yang H; Yanjing Medical College,Capital Medical University, Beijing 101300, China. Electronic address: yanghong@ccmu.edu.cn.
Fitoterapia ; 177: 106079, 2024 Sep.
Article em En | MEDLINE | ID: mdl-38897252
ABSTRACT
This study aims to elucidate the potential targets and molecular mechanisms underlying the anticancer effects of Red fermented rice extract using molecular simulation techniques. The inhibitory effects of different elution fractions of Red fermented rice extract on A549 and MCF-7 cell proliferation were evaluated through CCK-8 assays. Liquid chromatography-mass spectrometry (LC-MS) was employed to elucidate the structural information of active components, while molecular simulation techniques aided in identifying target proteins based on small molecule structures. Protein immunoblotting was utilized to investigate the mechanisms of action of relevant targets. The study found that the petroleum ether-ethyl acetate and ethyl acetate elution fractions of Red fermented rice extract significantly inhibited A549 and MCF-7 cell proliferation, with stronger effects observed on A549 cells. LC-MS structural analysis identified 25 small molecule structures. Molecular simulations successfully revealed interaction between active elution fractions of Red fermented rice extract and the cancer-related protein FGFR1. Further investigation into the phosphorylation of FGFR1 and its downstream pathway targets PI3K/AKT demonstrated that the active elution fractions exerted their anticancer activity by inhibiting the phosphorylation of FGFR1, PI3K, and AKT proteins. This comprehensive study, integrating CCK-8 assays, LC-MS, molecular simulation techniques, and protein immunoblotting, provides a deep understanding of the anticancer mechanisms of Red fermented rice extract, guiding its further development and clinical application.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oryza / Extratos Vegetais / Transdução de Sinais / Fosfatidilinositol 3-Quinases / Proliferação de Células / Proteínas Proto-Oncogênicas c-akt / Receptor Tipo 1 de Fator de Crescimento de Fibroblastos / Fermentação Limite: Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oryza / Extratos Vegetais / Transdução de Sinais / Fosfatidilinositol 3-Quinases / Proliferação de Células / Proteínas Proto-Oncogênicas c-akt / Receptor Tipo 1 de Fator de Crescimento de Fibroblastos / Fermentação Limite: Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article