LC/MS-guided isolation and GNPS procedures to identify flavonoid of HCoV-OC43 inhibitors.
Fitoterapia
; 177: 106077, 2024 Sep.
Article
em En
| MEDLINE
| ID: mdl-38906387
ABSTRACT
The screening of based target compounds supported by LC/MS, MS/MS and Global Natural Products Social (GNPS) used to identify the compounds 1-10 of Butea monsperma. They were evaluated in human malignant embryonic rhabdomyoma cells (RD cells) infected with Human coronavirus OC43 (HCoV-OC43) and showed significant inhibitory activity. Target inhibition tests showed that compounds 6 and 8 inhibited the proteolytic enzyme 3CLpro, which is widely present in coronavirus and plays an important role in the replication process, with an effective IC50 value. The study confirmed that dioxymethylene of compound 8 may be a key active fragment in inhibiting coronavirus (EC50 7.2 µM, SI > 139.1). The results have led to identifying natural bioactive compounds for possible inhibiting HCoV-OC43 and developing drug for Traditional Chinese Medicine (TCM).
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Antivirais
/
Flavonoides
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Coronavirus Humano OC43
Limite:
Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article