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An ultra low-input method for global RNA structure probing uncovers Regnase-1-mediated regulation in macrophages.
Piao, Meiling; Li, Pan; Zeng, Xiaomin; Wang, Xi-Wen; Kang, Lan; Zhang, Jinsong; Wei, Yifan; Zhang, Shaojun; Tang, Lei; Zhu, Jianghui; Kwok, Chun Kit; Hu, Xiaoyu; Zhang, Qiangfeng Cliff.
Afiliação
  • Piao M; MOE Key Laboratory of Bioinformatics, Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
  • Li P; Beijing Advanced Innovation Center for Structural Biology & Frontier Research Center for Biological Structure, School of Life Sciences, Tsinghua University, Beijing 100084, China.
  • Zeng X; Tsinghua-Peking Center for Life Sciences, Beijing 100084, China.
  • Wang XW; MOE Key Laboratory of Bioinformatics, Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
  • Kang L; Beijing Advanced Innovation Center for Structural Biology & Frontier Research Center for Biological Structure, School of Life Sciences, Tsinghua University, Beijing 100084, China.
  • Zhang J; Tsinghua-Peking Center for Life Sciences, Beijing 100084, China.
  • Wei Y; Institute for Immunology and School of Medicine, Tsinghua University, Beijing, China.
  • Zhang S; Beijing Key Laboratory for Immunological Research on Chronic Diseases, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, China.
  • Tang L; MOE Key Laboratory of Bioinformatics, Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
  • Zhu J; Beijing Advanced Innovation Center for Structural Biology & Frontier Research Center for Biological Structure, School of Life Sciences, Tsinghua University, Beijing 100084, China.
  • Kwok CK; Tsinghua-Peking Center for Life Sciences, Beijing 100084, China.
  • Hu X; Institute for Immunology and School of Medicine, Tsinghua University, Beijing, China.
  • Zhang QC; Beijing Key Laboratory for Immunological Research on Chronic Diseases, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, China.
Fundam Res ; 2(1): 2-13, 2022 Jan.
Article em En | MEDLINE | ID: mdl-38933905
ABSTRACT
To enable diverse functions and precise regulation, an RNA sequence often folds into complex yet distinct structures in different cellular states. Probing RNA in its native environment is essential to uncovering RNA structures of biological contexts. However, current methods generally require large amounts of input RNA and are challenging for physiologically relevant use. Here, we report smartSHAPE, a new RNA structure probing method that requires very low amounts of RNA input due to the largely reduced artefact of probing signals and increased efficiency of library construction. Using smartSHAPE, we showcased the profiling of the RNA structure landscape of mouse intestinal macrophages upon inflammation, and provided evidence that RNA conformational changes regulate immune responses. These results demonstrate that smartSHAPE can greatly expand the scope of RNA structure-based investigations in practical biological systems, and also provide a research paradigm for the study of post-transcriptional regulation.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2022 Tipo de documento: Article