Novel buffer for long-term preservation of DNA in biological material at room temperature.

ABSTRACT

The collection and preservation of biological material before DNA analysis is critical for inter alia biomedical research, medical diagnostics, forensics and biodiversity conservation. In this study, we evaluate an in-house formulated buffer called the Forensic DNA Laboratory-buffer (FDL-buffer) for preservation of biological material for long term at room temperature. Human saliva stored in the buffer for 8 years, human blood stored for 3 years and delicate animal tissues from the jellyfish Pelagia noctiluca comb jelly Beroe sp., stored for 4 and 6 years respectively consistently produced high-quality DNA. FDL-buffer exhibited compatibility with standard organic, salting out and spin-column extraction methods, making it versatile and applicable to a wide range of applications, including automation.

DNA extractions were performed by Salting out, PCI, ZymoQuick and DNAeazy methods. DNA quantity and quality were assessed using qPCR, Qubit, gel electrophoresis, as well as Sanger sequencing, microsatellite profiling and SNPchip analysis.