Comparative assessment of two in-house-built isothermal assays for visual detection of African swine fever virus.
J Biosci
; 492024.
Article
em En
| MEDLINE
| ID: mdl-38952078
ABSTRACT
Owing to the lack of effective vaccines, current control measures and eradication strategies for the African swine fever virus (ASFV) rely on early detection and stringent stamping-out procedures. In the present study, we developed two independent isothermal amplification assays, namely, loop-mediated isothermal amplification (LAMP) and polymerase spiral reaction (PSR), for quick visualization of the ASFV genome in clinical samples. Additionally, a quantitative real-time PCR (qRT-PCR)-based hydrolysis probe assay was developed for comparative assessment of sensitivity with the developed isothermal assays. The analytical sensitivity of the LAMP, PSR, and qRT-PCR was found to be 2.64 ×105 copies/µL, 2.64 ×102 copies/µL, and 2.64 ×101 copies/µL, respectively. A total of 165 clinical samples was tested using the developed visual assays. The relative accuracy, relative specificity, and relative diagnostic sensitivity for LAMP vs PSR were found to be 95.37% vs 102.48%, 97.46% vs 101.36%, and 73.33% vs 113.33%, respectively.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sensibilidade e Especificidade
/
Técnicas de Amplificação de Ácido Nucleico
/
Febre Suína Africana
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Vírus da Febre Suína Africana
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article