Elongatibacter sediminis gen. nov., sp. nov., isolated from intertidal sediment, and genomic comparison with all genera in the family Wenzhouxiangellaceae.
Int J Syst Evol Microbiol
; 74(7)2024 Jul.
Article
em En
| MEDLINE
| ID: mdl-38958649
ABSTRACT
A novel slightly halophilic, aerobic, and Gram-stain-negative strain, designated as CH-27T, was isolated during a bacterial resource investigation of intertidal sediment collected from Xiaoshi Island in Weihai, PR China. Cells of strain CH-27T were rod-shaped with widths of 0.3-0.6 µm and lengths of 2.0-11.0 µm. Strain CH-27T grew optimally at 37â°C, pH 7.0 and with 2.0â% (w/v) NaCl. Catalase activity was weakly positive and oxidase activity was positive. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CH-27T was most related to Marinihelvus fidelis KCTC 92639T (93.6â%), followed by Wenzhouxiangella marina MCCC 1K00261T (92.0â%). Based on genome comparisons between strain CH-27T and M. fidelis KCTC 92639T, the average amino acid identity was 63.6â% and the percentage of conserved proteins was 48.3â%. The major cellular fatty acid of strain CH-27T (≥10â%) was iso-C15ââ0 and the sole respiratory quinone was quinone-8. The polar lipids were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, and aminophospholipid. The DNA G+C content was 62.7âmol%. Based on comprehensive analysis of its phylogenetic, physiological, biochemical, and chemotaxonomic characteristics, strain CH-27T represents a novel species in a novel genus, for which the name Elongatibacter sediminis gen. nov., sp.nov. is proposed. The type strain is CH-27T (=MCCC 1H00480T=KCTC 8011T).
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Filogenia
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Composição de Bases
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DNA Bacteriano
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RNA Ribossômico 16S
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Técnicas de Tipagem Bacteriana
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Análise de Sequência de DNA
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Sedimentos Geológicos
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Ácidos Graxos
País/Região como assunto:
Asia
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article