Evaluation of the sensitivity of a measles diagnostic real-time RT-PCR assay incorporating recently observed priming mismatch variants, 2024.
Euro Surveill
; 29(28)2024 Jul.
Article
em En
| MEDLINE
| ID: mdl-38994600
ABSTRACT
We investigated a variant of measles virus that encodes three mismatches to the reverse priming site for a widely used diagnostic real-time RT-PCR assay; reduction of sensitivity was hypothesised. We examined performance of the assay in context of the variant using in silico data, synthetic RNA templates and clinical specimens. Sensitivity was reduced observed at low copy numbers for templates encoding the variant sequence. We designed and tested an alternate priming strategy, rescuing the sensitivity of the assay.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA Viral
/
Sensibilidade e Especificidade
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
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Reação em Cadeia da Polimerase em Tempo Real
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Sarampo
/
Vírus do Sarampo
Limite:
Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article