Ultra-Performance Liquid Chromatography (UPLC) Analysis of Heme Biosynthesis Intermediates.
Methods Mol Biol
; 2839: 213-223, 2024.
Article
em En
| MEDLINE
| ID: mdl-39008255
ABSTRACT
The utilization of ultra-performance liquid chromatography (UPLC) to analyze the various intermediates in the heme biosynthetic pathway is presented. The first product, ALA, was derivatized to a highly fluorescent pyrrolizine; PBG, the second intermediate, was enzymatically converted to uroporphyrinogen, and all the porphyrinogen intermediates were oxidized in acid to form fluorescent porphyrins. Heme was measured as hemin. The stable porphyrin forms of the intermediates, are then resolved and quantified by UPLC. Further details about the various methods are discussed to promote successful UPLC analyses. Method variations that may be preferable in certain situations are also presented.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Heme
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article