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Integrated transcriptomics of human blood vessels defines a spatially controlled niche for early mesenchymal progenitor cells.
Wang, Yiyun; Thottappillil, Neelima; Gomez-Salazar, Mario; Tower, Robert J; Qin, Qizhi; Del Rosario Alvia, Ishbel Camila; Xu, Mingxin; Cherief, Masnsen; Cheng, Ray; Archer, Mary; Arondekar, Shreya; Reddy, Sashank; Broderick, Kristen; Péault, Bruno; James, Aaron W.
Afiliação
  • Wang Y; Department of Pathology, Johns Hopkins University, Baltimore, MD 21205, USA.
  • Thottappillil N; Department of Pathology, Johns Hopkins University, Baltimore, MD 21205, USA.
  • Gomez-Salazar M; Department of Pathology, Johns Hopkins University, Baltimore, MD 21205, USA.
  • Tower RJ; Department of Surgery, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
  • Qin Q; Department of Pathology, Johns Hopkins University, Baltimore, MD 21205, USA.
  • Del Rosario Alvia IC; Department of Pathology, Johns Hopkins University, Baltimore, MD 21205, USA.
  • Xu M; Department of Pathology, Johns Hopkins University, Baltimore, MD 21205, USA.
  • Cherief M; Department of Pathology, Johns Hopkins University, Baltimore, MD 21205, USA.
  • Cheng R; Department of Pathology, Johns Hopkins University, Baltimore, MD 21205, USA.
  • Archer M; Department of Pathology, Johns Hopkins University, Baltimore, MD 21205, USA.
  • Arondekar S; Department of Pathology, Johns Hopkins University, Baltimore, MD 21205, USA.
  • Reddy S; Department of Plastic and Reconstructive Surgery, Johns Hopkins University, Baltimore, MD 21205, USA.
  • Broderick K; Department of Plastic and Reconstructive Surgery, Johns Hopkins University, Baltimore, MD 21205, USA.
  • Péault B; Department of Orthopedic Surgery and Orthopedic Hospital Research Center, University of California, Los Angeles, Los Angeles, CA 90095, USA.
  • James AW; Department of Pathology, Johns Hopkins University, Baltimore, MD 21205, USA. Electronic address: awjames@jhmi.edu.
Dev Cell ; 2024 Jul 15.
Article em En | MEDLINE | ID: mdl-39025061
ABSTRACT
Human blood vessel walls show concentric layers, with the outermost tunica adventitia harboring mesenchymal progenitor cells. These progenitor cells maintain vessel homeostasis and provide a robust cell source for cell-based therapies. However, human adventitial stem cell niche has not been studied in detail. Here, using spatial and single-cell transcriptomics, we characterized the phenotype, potential, and microanatomic distribution of human perivascular progenitors. Initially, spatial transcriptomics identified heterogeneity between perivascular layers of arteries and veins and delineated the tunica adventitia into inner and outer layers. From this spatial atlas, we inferred a hierarchy of mesenchymal progenitors dictated by a more primitive cell with a high surface expression of CD201 (PROCR). When isolated from humans and mice, CD201Low expression typified a mesodermal committed subset with higher osteogenesis and less proliferation than CD201High cells, with a downstream effect on canonical Wnt signaling through DACT2. CD201Low cells also displayed high translational potential for bone tissue generation.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2024 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2024 Tipo de documento: Article