Olink Proteomics-Based Exploration of Immuno-Oncology-Related Biomarkers Leading to Lung Adenocarcinoma Progression.
J Proteome Res
; 23(8): 3674-3681, 2024 Aug 02.
Article
em En
| MEDLINE
| ID: mdl-39028944
ABSTRACT
INTRODUCTION:
It is crucial to investigate the distinct proteins that contribute to the advancement of lung cancer. MATERIAL ANDMETHODS:
We analyzed the expression levels of 92 immuno-oncology-related proteins in 96 pairs of lung adenocarcinoma tissue samples using Olink proteomics. The differentially expressed proteins (DEPs) were successively screened in tumor and paraneoplastic groups, early and intermediate-late groups by a nonparametric rank sum test, and the distribution and expression levels of DEPs were determined by volcano and heat maps, etc., and the area under the curve was calculated.RESULTS:
A total of 24 DEPs were identified in comparisons between tumor and paracancerous tissues. Among them, interleukin-8 (IL8) and chemokine (C-C motif) ligand 20 (CCL20) as potential markers for distinguishing tumor tissues. Through further screening, it was found that interleukin-6 (IL6) and vascular endothelial growth factor A (VEGFA) may be able to lead to tumor progression through the JaK-STAT signaling pathway, Toll-like receptor signaling pathway and PI3K/AKT signaling pathway. Interestingly, our study revealed a down-regulation of IL6 and VEGFA in tumor tissues compared to paracancerous tissues.CONCLUSIONS:
IL8 + CCL20 (AUC 0.7056) have the potential to differentiate tumor tissue from paracancerous tissue; IL6 + VEGFA (AUC 0.7531) are important protein markers potentially responsible for tumor progression.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Biomarcadores Tumorais
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Interleucina-8
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Progressão da Doença
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Proteômica
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Fator A de Crescimento do Endotélio Vascular
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Quimiocina CCL20
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Adenocarcinoma de Pulmão
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Neoplasias Pulmonares
Limite:
Female
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Humans
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Male
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article