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Identification of RVFV Host Factors Using Quantitative Interaction Proteomics.
Wilken, Lucas; Lasswitz, Lisa; Scaturro, Pietro; Gerold, Gisa.
Afiliação
  • Wilken L; Leibniz Institute of Virology (LIV), Hamburg, Germany.
  • Lasswitz L; Institute of Biochemistry & Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Hannover, Germany.
  • Scaturro P; Leibniz Institute of Virology (LIV), Hamburg, Germany. pietro.scaturro@leibniz-liv.de.
  • Gerold G; Institute of Biochemistry & Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Hannover, Germany. gisa.gerold@tiho-hannover.de.
Methods Mol Biol ; 2824: 189-202, 2024.
Article em En | MEDLINE | ID: mdl-39039414
ABSTRACT
Affinity enrichment coupled with liquid chromatography-tandem mass spectrometry (AE-LC-MS/MS) enables a comprehensive study of virus-host protein-protein interactions in cells and tissues infected with Rift Valley fever virus (RVFV) or ectopically expressing RVFV proteins. Depending on the research question, different experimental setups with carefully chosen controls are needed. Here, we describe the detailed workflow of sample preparation, processing, and cleanup, while also outlining critical points to consider when designing and performing AE-LC-MS/MS experiments.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vírus da Febre do Vale do Rift / Proteômica / Espectrometria de Massas em Tandem / Interações Hospedeiro-Patógeno Limite: Animals / Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vírus da Febre do Vale do Rift / Proteômica / Espectrometria de Massas em Tandem / Interações Hospedeiro-Patógeno Limite: Animals / Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article