Identification of RVFV Host Factors Using Quantitative Interaction Proteomics.
Methods Mol Biol
; 2824: 189-202, 2024.
Article
em En
| MEDLINE
| ID: mdl-39039414
ABSTRACT
Affinity enrichment coupled with liquid chromatography-tandem mass spectrometry (AE-LC-MS/MS) enables a comprehensive study of virus-host protein-protein interactions in cells and tissues infected with Rift Valley fever virus (RVFV) or ectopically expressing RVFV proteins. Depending on the research question, different experimental setups with carefully chosen controls are needed. Here, we describe the detailed workflow of sample preparation, processing, and cleanup, while also outlining critical points to consider when designing and performing AE-LC-MS/MS experiments.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vírus da Febre do Vale do Rift
/
Proteômica
/
Espectrometria de Massas em Tandem
/
Interações Hospedeiro-Patógeno
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article